FISH, CLL/MM, D13S319, Deletion 13q14.3

Test Code
16670


CPT Codes
88271 (x2), 88275

Preferred Specimen
3 mL bone marrow or 5 mL whole blood collected in sodium heparin (green-top) tube


Minimum Volume
1 mL bone marrow • 3 mL whole blood


Other Acceptable Specimens
Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube • 5x5mm fresh lymph node collected in Hanks' or Ringer's solution


Instructions
Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.
Submit 1 to 3 mL of bone marrow or 3 to 5 mL whole blood in a sodium heparin (green-top) tube.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.


Transport Temperature
Room temperature


Specimen Stability
Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions


Methodology
Fluorescence in situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Monday-Sunday Morning
Report available: 5 Days


Reference Range
See Laboratory Report


Clinical Significance
This test is performed in addition to G-banding chromosome analysis tests for bone marrow, hematologic blood, or lymph node. Deletion of chromosome 13q14.3 occurs in multiple myeloma, non Hodgkins lymphoma, chronic lymphocytic leukemia, myeloproliferative disorders, myelodysplastic syndrome, and acute myelogenous leukemia. The D13S319 Fluorescence In Situ Hybridization (FISH) probe is useful in detecting deletions in a higher percentage of cells than by routine cytogenetic studies. The probe can be used to evaluate complex karyotypes for loss of this critical gene region.




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.