FISH, Lymphoid Disorder, 6q Deletion

Test Code

16593

CPT Codes
88271, 88275

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Other Acceptable Specimens

Bone marrow or whole blood collected in: sodium heparin (royal blue-top) tube, sodium heparin lead-free (tan-top) tube • 5x5 mm lymph node submitted in culture transport medium • Bone marrow in culture transport medium

Instructions

Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.

Specimen viability decreases during transit. Send specimen to testing laboratory for viability determination. Do not freeze. Do not reject.

Note: 100-300 interphase cells are microscopically analyzed for probe signal patterns, indicating the copy number for the MYB region of chromosome 6q23.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions

Methodology
Fluorescence in situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Sun-Fri; Report available: 5 days

Reference Range

See Laboratory Report

Clinical Significance

Chromosome 6q deletion is a frequent abnormality in lymphoid malignancies including acute lymphoblastic leukemia, chronic lymphoid disorders, non-Hodgkin lymphoma, and multiple myeloma. It may be the sole clonal abnormality in as much as 30% of cases. Deletions may be small and difficult to resolve using conventional chromosome analysis. Deletion of MYB may be associated with loss of recessive tumor suppressor gene and may be an initial step in leukemogenesis.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.