FISH, Ewing/PNET, EWSR1, 22q12 Rearrangements

Test Code

16112

CPT Codes
88275, 88271 (x2)

Preferred Specimen

Fresh-fixed, paraffin-embedded specimen or
3 mL bone marrow collected in a sodium heparin (green-top) tube or
5 x 5 mm tissue biopsy collected in transport medium

Minimum Volume

1 mL bone marrow

Instructions

Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Note: If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions

Methodology
Fluorescence in situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 5 days

Reference Range

Interpretive report

Clinical Significance

A FISH Ewing sarcoma assay by using the LSI (R) EWSR1 (22q12) Dual Color, Break Apart Rearrangement Probe (Vysis) to identify chromosomal rearrangements of the EWSR1 gene region.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.