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FISH, ALL/NHL, MYC-BA, 8q24 Rearrangement
Test Code14706
CPT Codes
88271 (x2), 88275
Preferred Specimen
5 x 5 mm lymph node collected in a sterile container in Hanks' Ringers solution or culture medium with antibiotics
Minimum Volume
5x5 mm lymph node • 1 mL bone marrow • 3 mL whole blood
Other Acceptable Specimens
3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) or sodium heparin lead-free (tan-top) • Formalin-fixed, paraffin-embedded tissue block or slides • Fresh (unfixed) tissue
Instructions
Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.
For bone marrow (1-3 mL) or whole blood (3-5 mL), use a green-top tube with sodium heparin. For lymph node biopsy, 5 x 5 mm in sterile container in Hanks' Ringers solution or culture medium with antibiotics. Transportation medium available upon request.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
For bone marrow (1-3 mL) or whole blood (3-5 mL), use a green-top tube with sodium heparin. For lymph node biopsy, 5 x 5 mm in sterile container in Hanks' Ringers solution or culture medium with antibiotics. Transportation medium available upon request.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions
Refrigerated: See instructions
Frozen: See instructions
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 5 days
Reference Range
See Laboratory Report
Clinical Significance
This fluorescence in situ hybridization (FISH) test is
used to identify rearrangements of the C-MYC region
located on chromosome band 8q24 using the a dual color
breakapart (BA) DNA probe. This rearrangement is
observed in a majority of Burkitt lymphomas.
100-200 interphase nuclei are examined microscopically
for the suspected gene rearrangement. This test is
useful for evaluation of suboptimal specimens or when a
variant or cryptic rearrangement is suspected.
used to identify rearrangements of the C-MYC region
located on chromosome band 8q24 using the a dual color
breakapart (BA) DNA probe. This rearrangement is
observed in a majority of Burkitt lymphomas.
100-200 interphase nuclei are examined microscopically
for the suspected gene rearrangement. This test is
useful for evaluation of suboptimal specimens or when a
variant or cryptic rearrangement is suspected.
