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FISH, High-Grade Lymphoma Panel
Test Code91606
CPT Codes
88275 (x3), 88271 (x6)
Includes
100-300 interphase cells are microscopically analyzed for each of the following probe sets: Rearrangements of MYC (8q24); Rearrangements of BCL6 (3q27); IGH/BCL2 t(14;18)
Preferred Specimen
3 mL bone marrow submitted in transport medium
Minimum Volume
1 mL bone marrow
Other Acceptable Specimens
Bone marrow collected in: Sodium heparin (green-top) tube, sodium heparin (royal blue-top) tube or sodium heparin (tan-top) tube • 5x5mm fresh lymph node/tumor biopsy in transport medium, or formalin-fixed paraffin-embedded tissue block
Instructions
Bone Marrow, 1-3 mL in transport medium (preferred) or sodium heparin (green-top, dark/royal blue-top or tan-top) tubes are acceptable containers for this test.
Lymph node/tumor: 5X5 mm fresh tumor biopsy in a transport medium.
Paraffin Block: Formalin-fixed, paraffin-embedded tissue.
Transport media available upon request.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Lymph node/tumor: 5X5 mm fresh tumor biopsy in a transport medium.
Paraffin Block: Formalin-fixed, paraffin-embedded tissue.
Transport media available upon request.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions
Refrigerated: See instructions
Frozen: See instructions
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Monday-Sunday Evening
Report available: 7 Days
Report available: 7 Days
Reference Range
See Laboratory Report
Clinical Significance
High-grade B-cell lymphoma can be difficult to classify if histologic and immunophenotypic features are not entirely typical. Large B-cell lymphoma with monomorphic appearance or very high mitotic rate and Burkitt lymphoma cases that have atypical immunophenotypic or morphologic appearances present diagnostic difficulties. The FISH panel for MYC (8q24), t(14;18) (IGH-BCL2) and BCL6 (3q27) translocations, provides additional diagnostic information that may help in final classification and therapy selection in high-grade B-cell lymphomas as well as to assess for "double-hit" lymphomas and "triple-hit" lymphomas.