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B-Cell Phenotyping Profile for Immunodeficiency and Immune Competence Assessment, Blood
Test Code92717
CPT Codes
86355, 86356 (x7), 86357, 86359, 86360
Preferred Specimen
TBBS/Quantitative Lymphocyte Subsets:
3 mL whole blood collected in an EDTA (lavender-top) tube
--and--
IABC/B-Cell Phenotyping Screen:
Ages > 14 years: 10 mL whole blood collected in an EDTA (lavender-top) tube, or
Ages ≤ 14 years: 4 mL whole blood collected in an EDTA (lavender-top) tube
3 mL whole blood collected in an EDTA (lavender-top) tube
--and--
IABC/B-Cell Phenotyping Screen:
Ages > 14 years: 10 mL whole blood collected in an EDTA (lavender-top) tube, or
Ages ≤ 14 years: 4 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
TBBS/Quantitative Lymphocyte Subsets: 1 mL
IABC/B-Cell Phenotyping Screen:
Ages > 14 years: 5 mL
Ages ≤ 14 years: 3 mL
IABC/B-Cell Phenotyping Screen:
Ages > 14 years: 5 mL
Ages ≤ 14 years: 3 mL
Instructions
Two separate Whole Blood EDTA specimens are required: 1 refrigerated and 1 ambient transport temperature.
Whole blood for TBBS/Quantitative Lymphocyte Subsets: T, B, and NK:
1.) Send specimen in original tube. Do not aliquot.
2.) Label specimen as blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and NK. Specimen Stability: Ambient
--AND--
Whole blood for IABC/B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood:
1.) Send specimen in original tube. Do not aliquot.
2.) Label specimen as blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood.
Specimen Stability: Refrigerated
Necessary Information:
1.) Date of draw is required.
2.) Ordering physician's name and phone number are required.
Shipping Instructions: Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible. It is recommended that specimens arrive within 24 hours of draw. Samples arriving on the weekend and observed holidays may be canceled. Client Direct Ship Process is required.
Whole blood for TBBS/Quantitative Lymphocyte Subsets: T, B, and NK:
1.) Send specimen in original tube. Do not aliquot.
2.) Label specimen as blood for TBBS / Quantitative Lymphocyte Subsets: T, B, and NK. Specimen Stability: Ambient
--AND--
Whole blood for IABC/B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood:
1.) Send specimen in original tube. Do not aliquot.
2.) Label specimen as blood for IABC / B-Cell Phenotyping Screen for Immunodeficiency and Immune Competence Assessment, Blood.
Specimen Stability: Refrigerated
Necessary Information:
1.) Date of draw is required.
2.) Ordering physician's name and phone number are required.
Shipping Instructions: Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible. It is recommended that specimens arrive within 24 hours of draw. Samples arriving on the weekend and observed holidays may be canceled. Client Direct Ship Process is required.
Transport Temperature
TBBS/Quantitative Lymphocyte Subsets: Room temperature
IABC/B-Cell Phenotyping Screen: Refrigerated (cold packs)
IABC/B-Cell Phenotyping Screen: Refrigerated (cold packs)
Specimen Stability
TBBS/Quantitative Lymphocyte Subsets
Room temperature: < 52 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
IABC/B-Cell Phenotyping Screen
Room temperature: Unacceptable
Refrigerated: < 48 hours
Frozen: Unacceptable
Room temperature: < 52 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
IABC/B-Cell Phenotyping Screen
Room temperature: Unacceptable
Refrigerated: < 48 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Gross lipemia
Methodology
TBBS: Flow Cytometry • IABC: Fluorescent Flow Cytometry
FDA Status
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
Setup Schedule
Set up: Mon-Fri; Report available: 3-4 days
Reference Range
See Laboratory Report
Clinical Significance
Screening for common variable immunodeficiency (CVID) and hyper-IgM syndromes Assessing B-cell subset reconstitution after stem cell or bone marrow transplant Assessing response to B-cell-depleting immunotherapy Identifying defects in transmembrane activator and calcium modulator and cyclophilin ligand (CAML) interactor (TACI) and B-cell-activating factor receptor (BAFF-R) in patients presenting with clinical symptoms and other laboratory features consistent with CVID.
