Factor VII Activity, Clotting

Test Code

346

CPT Codes
85230

Preferred Specimen

1 mL frozen platelet-poor plasma collected from a 3.2% sodium citrate (light blue-top) tube

Minimum Volume

0.5 mL

Instructions

Please submit a separate, frozen vial for each special coagulation assay ordered. Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Freeze immediately and transport on dry ice.

Transport Container

Transport tube

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis

Methodology
Photometric Clot Detection

Setup Schedule

Monday-Friday Evening Report available: Next Day

Limitations

Expected impact by therapeutic levels (potential interference depends upon drug concentration): Warfarin: decreased, Heparin (UFH or LMWH): no effect (reagent contains heparin neutralizer); Dabigatran or Argatroban (Thrombin Inhibitors): no effect to inhibitor pattern or falsely low; Rivaroxaban or Apixaban (Factor Xa Inhibitors): no effect to inhibitor pattern.

Reference Range

60-150 % normal

Clinical Significance

Test is useful to evaluate a prolonged PT. Deficiency is associated with bleeding risk.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.