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Bilirubin, Fractionated
Test Code7286
CPT Codes
82247, 82248
Includes
Direct Bilirubin, Indirect Bilirubin (calculated), Total Bilirubin
Preferred Specimen
1 mL serum collected in a serum separator tube (SST) - protect from light
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in: sodium heparin (green-top) tube, or lithium heparin (green-top) tube
Instructions
Protect from light: Please place in an amber vial or wrap plastic vial in foil; avoid hemolysis.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: 90 days
Refrigerated: 72 hours
Frozen: 90 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Anticoagulants other than heparin
Methodology
Spectrophotometry (SP)
Setup Schedule
Set up: Daily; Report available: Next day
Reference Range
Bilirubin, Direct | ≤0.2 | mg/dL | |||||||||||||||||||||
Bilirubin, Indirect | |||||||||||||||||||||||
≤1 Day | ≤5.1 | mg/dL (calc) | |||||||||||||||||||||
2 Days | ≤7.2 | mg/dL (calc) | |||||||||||||||||||||
3-5 Days | ≤10.3 | mg/dL (calc) | |||||||||||||||||||||
6-7 Days | ≤8.4 | mg/dL (calc) | |||||||||||||||||||||
8-9 Days | ≤6.5 | mg/dL (calc) | |||||||||||||||||||||
10-11 Days | ≤4.6 | mg/dL (calc) | |||||||||||||||||||||
12-13 Days | ≤2.7 | mg/dL (calc) | |||||||||||||||||||||
14 Days - 9 Years | 0.2-0.8 | mg/dL (calc) | |||||||||||||||||||||
10-19 Years | 0.2-1.1 | mg/dL (calc) | |||||||||||||||||||||
≥20 Years | 0.2-1.2 | mg/dL (calc) | |||||||||||||||||||||
Bilirubin, Total | |||||||||||||||||||||||
≤1 Day | ≤5.1 | mg/dL | |||||||||||||||||||||
2 Days | ≤7.2 | mg/dL | |||||||||||||||||||||
3-5 Days | ≤10.3 | mg/dL | |||||||||||||||||||||
6-7 Days | ≤8.4 | mg/dL | |||||||||||||||||||||
8-9 Days | ≤6.5 | mg/dL | |||||||||||||||||||||
10-11 Days | ≤4.6 | mg/dL | |||||||||||||||||||||
12-13 Days | ≤2.7 | mg/dL | |||||||||||||||||||||
14 Days - 9 Years | 0.2-0.8 | mg/dL | |||||||||||||||||||||
10-19 Years | 0.2-1.1 | mg/dL | |||||||||||||||||||||
≥20 Years | 0.2-1.2 | mg/dL |
Clinical Significance
Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder obstructive disease.