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Insulin Response to Glucose, 3 Specimens
Test Code6695
CPT Codes
83525 (x3)
Preferred Specimen
1 mL serum (x3)
Patient Preparation
Overnight fasting is required.
High carbohydrate diet for 3 days before test. Patient NPO after midnight; glucose solution 1.75 g/kg body weight (maximum dose 75 g) orally administered after collection of fasting specimen.
Minimum Volume
0.5 mL (x3)
Instructions
Draw fasting specimen. Collect 1 mL serum for each timed specimen post glucose dose. All tubes must be clearly marked with time drawn. Submit all tubes with one test requisition.
Reference ranges available: Fasting and 30, 60, 90, 120, 150, 180, 240 and 300 minutes post glucose.
Unable to add INSULIN testing unless sample was submitted frozen.
Reference ranges available: Fasting and 30, 60, 90, 120, 150, 180, 240 and 300 minutes post glucose.
Unable to add INSULIN testing unless sample was submitted frozen.
Transport Container
Transport tube(s)
Transport Temperature
Refrigerated (cold Packs)
Specimen Stability
Room temperature: 8 hours
Refrigerated: 7 days
Frozen: 28 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Plasma • Grossly icteric
Methodology
Immunoassay (IA)
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Reference Range
| Fasting | 2.0-18.4 uIU/mL |
| 30 Minutes Post Glucose | 6.0-86.0 uIU/mL |
| 60 Minutes Post Glucose | 8.0-112.0 uIU/mL |
| 90 Minutes Post Glucose | 5.0-68.0 uIU/mL |
| 120 Minutes Post Glucose | 5.0-55.0 uIU/mL |
| 150 Minutes Post Glucose | 3.0-46.0 uIU/mL |
| 180 Minutes Post Glucose | 3.0-20.0 uIU/mL |
| 240 Minutes Post Glucose | <15.0 uIU/mL |
| 300 Minutes Post Glucose | <8.0 uIU/mL |
These values are provided for general guidance only.
Clinical Significance
The insulin response to glucose infusion is useful in evaluating patients with hypoglycemia and suspected insulin-resistance.
