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Bilirubin, Total
Test Code287
CPT Codes
82247
Preferred Specimen
1 mL serum collected in a serum separator tube (SST) - protected from light
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in: sodium heparin (green-top) tube, or lithium heparin (green-top) tube - protected from light
Instructions
Protect from light by wrapping a spun SST in foil or transferring serum or plasma to an amber transport vial. Avoid hemolysis.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 72 hours
Refrigerated: 72 hours
Frozen: 90 days
Refrigerated: 72 hours
Frozen: 90 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Anticoagulants other than heparin
Methodology
Spectrophotometry (SP)
Setup Schedule
Set up: Daily; Report available: Next day
Reference Range
| ≤1 Day | ≤5.1 | mg/dL |
| 2 Days | ≤7.2 | mg/dL |
| 3-5 Days | ≤10.3 | mg/dL |
| 6-7 Days | ≤8.4 | mg/dL |
| 8-9 Days | ≤6.5 | mg/dL |
| 10-11 Days | ≤4.6 | mg/dL |
| 12-13 Days | ≤2.7 | mg/dL |
| 14 Days-9 Years | 0.2-0.8 | mg/dL |
| 10-19 Years | 0.2-1.1 | mg/dL |
| ≥20 Years | 0.2-1.2 | mg/dL |
Clinical Significance
Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gallbladder obstructive disease.
