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Bilirubin, Direct
Test Code285
CPT Codes
82248
Preferred Specimen
1 mL serum collected in a serum separator tube (SST), spun - protected from light
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in: sodium heparin (green-top) tube, or lithium heparin (green-top) tube - protected from light • Serum collected in an amber transport vial - protected from light
Instructions
Please note: this test code should not be used for patients 2 months or younger.
Protect from light by wrapping a spun SST® in foil or transferring serum/plasma to an amber transport vial. Avoid hemolysis.
Protect from light by wrapping a spun SST® in foil or transferring serum/plasma to an amber transport vial. Avoid hemolysis.
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: 90 days
Refrigerated: 72 hours
Frozen: 90 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Anticoagulants other than heparin
Methodology
Spectrophotometry (SP)
Setup Schedule
Monday-Friday All Shifts Saturday Morning Sunday Morning, Night Report available: Next Day
Reference Range
≤0.2 mg/dL
Clinical Significance
Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder obstruction. The assessment of direct bilirubin is helpful in the differentiation of hepatic disorders. The increase in total bilirubin associated with obstructive jaundice is primarily due to the direct (conjugated) fraction. Both direct and indirect bilirubin are increased in the serum with hepatitis.
