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Enterovirus RNA, Quantitative Real-Time PCR
Test Code37936
CPT Codes
87799
Preferred Specimen
0.7 mL serum or
0.7 mL plasma collected in EDTA (lavender-top) or ACD (yellow-top) tube or
0.7 mL CSF collected in a sterile, screw-cap container
0.7 mL plasma collected in EDTA (lavender-top) or ACD (yellow-top) tube or
0.7 mL CSF collected in a sterile, screw-cap container
Minimum Volume
0.3 mL
Instructions
To avoid contamination, no aliquot is ever returned to the original container.
Transport Container
Plastic screw-cap container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens containing heparin
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1-3 days
Reference Range
| Enterovirus RNA, QN RT PCR | No RNA Detected (copies/mL) |
Clinical Significance
The enterovirus (EV) RNA quantitative PCR is a highly sensitive method used to detect and quantify enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human enteroviruses, including the coxsackieviruses, polioviruses and the echoviruses. Enterovirus D68 is detected. However, this assay does not differentiate among the EV serotypes.
