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CLL/Lymphoma Follow-up Panel (NY)
Test Code37527
CPT Codes
88184, 88188, 88185 (x14)<br /> <strong>This test code is for New York patient testing. For non-New York patient testing, use test code 17818. </strong>
Includes
Initial markers evaluated: CD3, CD4, CD5, CD7, CD8, CD10, CD11c, CD19, CD20, CD23, CD38, HLA-DR, sKappa, sLambda. CD45 is used for gating.
Additional markers may be performed based on the pathologist review. These markers will be performed at an additional charge (CPT code(s): 88185 for each additional marker)
Additional markers may be performed based on the pathologist review. These markers will be performed at an additional charge (CPT code(s): 88185 for each additional marker)
Preferred Specimen
Peripheral blood: 5 mL collected in a sodium heparin (green- top) tube or EDTA (lavender-top) tube
Bone marrow: 4 mL collected in a sodium heparin (green-top) or EDTA (lavender-top) tube
Tissue or body fluids: Any type is acceptable.
Bone marrow: 4 mL collected in a sodium heparin (green-top) or EDTA (lavender-top) tube
Tissue or body fluids: Any type is acceptable.
Minimum Volume
3 mL peripheral blood • 1 mL bone marrow
Instructions
Peripheral blood and bone marrow: The tube must be kept at room temperature and shipped to the lab immediately.
Tissue: Any tissue type is acceptable. Tissue size is dependent upon leukocyte cellularity. (The tissue is disaggregated into single cells so that a minimum of 50,000 cells of interest are harvested.) Ship tissue in a sterile plastic container with RPMI 1640 enriched with FBS (10% FBS RPMI). Absolutely no fixative should be added. Refrigerate and ship immediately.
Body fluids: Any body fluid is acceptable. Sample size is dependent upon cellularity of the sample. (A minimum of 50,000 cells of interest in total volume of fluid). Place fluid in sterile plastic container. Absolutely no fixative should be added. Refrigerate and ship immediately.
A clinical indication and specimen source are required with each specimen. If possible, submit CBC results with differential or an EDTA tube of peripheral blood.
Do not freeze and do not place in fixative.
Because of the critical nature of these specimens, the laboratory will attempt to process all specimens received, regardless of age of specimen.
Tissue: Any tissue type is acceptable. Tissue size is dependent upon leukocyte cellularity. (The tissue is disaggregated into single cells so that a minimum of 50,000 cells of interest are harvested.) Ship tissue in a sterile plastic container with RPMI 1640 enriched with FBS (10% FBS RPMI). Absolutely no fixative should be added. Refrigerate and ship immediately.
Body fluids: Any body fluid is acceptable. Sample size is dependent upon cellularity of the sample. (A minimum of 50,000 cells of interest in total volume of fluid). Place fluid in sterile plastic container. Absolutely no fixative should be added. Refrigerate and ship immediately.
A clinical indication and specimen source are required with each specimen. If possible, submit CBC results with differential or an EDTA tube of peripheral blood.
Do not freeze and do not place in fixative.
Because of the critical nature of these specimens, the laboratory will attempt to process all specimens received, regardless of age of specimen.
Transport Container
See Collection Instructions
Transport Temperature
Peripheral blood and bone marrow: Room temperature
Tissue and body fluids: Refrigerated (cold packs)
Tissue and body fluids: Refrigerated (cold packs)
Specimen Stability
Sodium heparin (green-top) tube
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
EDTA (lavender-top) tube
Room temperature: 30 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Tissue
Room temperature: 24 hours
Refrigerated: 48 hours
Frozen: Unacceptable
Body fluids
Room temperature: 24 hours
Refrigerated: 24 hours
Frozen: Unacceptable
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
EDTA (lavender-top) tube
Room temperature: 30 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Tissue
Room temperature: 24 hours
Refrigerated: 48 hours
Frozen: Unacceptable
Body fluids
Room temperature: 24 hours
Refrigerated: 24 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
See instructions
Methodology
Flow Cytometry (FC)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 2 days
Reference Range
See Laboratory Report
Clinical Significance
To aid in the diagnosis of leukemia or lymphoma.
Initial markers evaluated: CD3, CD4, CD5, CD7, CD8, CD10, CD11c, CD19, CD20, CD23, CD38, HLA-DR, sKappa, sLambda. CD45 is used for gating.
Initial markers evaluated: CD3, CD4, CD5, CD7, CD8, CD10, CD11c, CD19, CD20, CD23, CD38, HLA-DR, sKappa, sLambda. CD45 is used for gating.