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BK and JC Virus DNA, Qualitative Real-Time PCR
Test Code11068
CPT Codes
87798 (x2)
Preferred Specimen
0.7 mL random clean catch urine collected in a sterile plastic leak-proof container, or
0.7 mL plasma collected in a PPT (white-top) tube, or EDTA (lavender-top) tube, or ACD (yellow-top) tube
0.7 mL plasma collected in a PPT (white-top) tube, or EDTA (lavender-top) tube, or ACD (yellow-top) tube
Minimum Volume
0.3 mL
Other Acceptable Specimens
CSF collected in a sterile plastic leak-proof CSF container • Serum
Instructions
Urine and CSF: Collect in a sterile, plastic, leak-proof container. Freeze and ship frozen.
Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant. Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap, aliquot tubes.
Serum: Collect blood in sterile tubes with no anticoagulants; (SS, serum separator tubes) are recommended. Allow blood to clot at room temperature and separate serum from cells within 1 hour of collection. Transfer serum to sterile, plastic, screw-capped aliquot tubes. Freeze and ship frozen.
Plasma: Collect blood in sterile tubes containing EDTA or ACD as anticoagulant. Store collected whole blood at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-cap, aliquot tubes.
Serum: Collect blood in sterile tubes with no anticoagulants; (SS, serum separator tubes) are recommended. Allow blood to clot at room temperature and separate serum from cells within 1 hour of collection. Transfer serum to sterile, plastic, screw-capped aliquot tubes. Freeze and ship frozen.
Transport Container
Sterile, plastic screw-cap container or tubes
Transport Temperature
Frozen
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Plasma samples collected with anticoagulants other than EDTA and ACD • Whole blood
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Monday-Sunday Morning
Report available: Next Day
Report available: Next Day
Reference Range
Not Detected
Clinical Significance
This test is used to determine the presence of BK and JC Virus DNA in patient's specimens. Detection of the virus in these specimens may be indicative of an active infection, as PCR detects the presence of the virus, rather than the host's reaction to the virus.
