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14-3-3 Protein, CSF (Prion Disease)
Test Code37989
CPT Codes
0035U, 83520, 84394
Preferred Specimen
2 mL frozen CSF collected in a sterile screw-cap vial
Minimum Volume
1 mL
Other Acceptable Specimens
Frozen CSF collected in a Sarstedt CSF false-bottom tube
Instructions
The patient must be 12 years of age or older.
Cerebrospinal Fluid (CSF) obtained by lumbar puncture. The first 2 mL of CSF that flows from the tap should be discarded. Collect 2 mL of CSF (1 mL minimum), avoiding bloody tap. The sample must be immediately frozen, at least in a -20° C freezer. Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours).
Required patient information: Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.
*** Ship Monday through Wednesday (priority overnight) ***
Cerebrospinal Fluid (CSF) obtained by lumbar puncture. The first 2 mL of CSF that flows from the tap should be discarded. Collect 2 mL of CSF (1 mL minimum), avoiding bloody tap. The sample must be immediately frozen, at least in a -20° C freezer. Freeze within 20 minutes of collection. Store and transport frozen. Ship using a styrofoam container with sufficient dry ice (5 lbs/24 hours).
Required patient information: Please complete and send the National Prion Requisition "Test Request Form", available on the website at National Prion Disease Pathology Surveillance Center with the sample.
*** Ship Monday through Wednesday (priority overnight) ***
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: Indefinitely
Refrigerated: Unacceptable
Frozen: Indefinitely
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Bloody and insufficient sample quantity • Samples that are colored or contain blood cannot be performed for RT-QuIC and 14-3-3 GAMMA
Methodology
Immunoassay • RT-QuIC
Setup Schedule
Set up: Varies; Report available: 7 days
Reference Range
See Laboratory Report
Clinical Significance
In CSF: Search for the presence of the 14-3-3 protein. The 14-3-3 protein is a marker for some prion diseases, such as Creutzfeldt-Jakob disease (CJD), when a number of other neurodegenerative conditions are excluded.
