|
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
MGMT Promoter Methylation Tumor
Test Code37374
CPT Codes
81287
Preferred Specimen
Formalin-fixed paraffin-embedded tumor tissue block
Other Acceptable Specimens
5 unstained (minimum 5-micron thick sections) tumor slides (non-baked), and 1 H&E stained slide
Instructions
At least 40% tumor is required for this assay. In general, a 6 mm x 3 mm area of tissue cut at 5-micron thickness is the minimum amount of tissue needed. Tissue should be collected over multiple slides.
Note: When this test is ordered, slide review will always be performed at an additional charge (CPT 88381). Pathology report must accompany specimen in order for testing to be performed.
Submit one slide stained with hematoxylin and eosin and five unstained, nonbaked 5-micron thick sections of tumor
Note: When this test is ordered, slide review will always be performed at an additional charge (CPT 88381). Pathology report must accompany specimen in order for testing to be performed.
Submit one slide stained with hematoxylin and eosin and five unstained, nonbaked 5-micron thick sections of tumor
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Varies
Refrigerated: Not established
Frozen: Not established
Refrigerated: Not established
Frozen: Not established
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens that have been decalcified (all methods) • Specimens that have not been formalin-fixed, paraffin-embedded • Bone marrow in EDTA
Methodology
Methylation-Specific Polymerase Chain Reaction (PCR) Analysis
Setup Schedule
Set up: Varies (weekly); Report available: 7-10 days
Report Available
10 Days
Reference Range
Interpretive report
Clinical Significance
Prognostication of newly diagnosed glioblastomas. Identifying newly diagnosed glioblastomas that may respond to alkylating chemotherapy (ie, temozolomide). Guiding therapy decision making for newly diagnosed glioblastomas in elderly patients (>60 years).