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Cryoglobulin, Qualitative Analysis
Test Code383
CPT Codes
82595
Preferred Specimen
3 mL fasting serum collected in a red-top tube (no gel)
Patient Preparation
Overnight fasting is required
Minimum Volume
2 mL
Instructions
Collect 6 mL of fasting whole blood specimen in a red-top tube (no gel). 3 mL serum required: 2 mL minimum. Avoid hemolysis.
Allow serum sample to clot for one (1) hour in a 37° C water bath, oven, incubator, or heat block. After clotting, centrifuge the specimen for 10 minutes at 1600 RCF(G) utilizing one of the 3 options below:
1) In a "warm" centrifuge at 37° C.
2) In a bench-top centrifuge placed in a "warm room" at 37° C.
3) At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 1600 RCF(G).
After centrifuging, decant serum into a screw-cap vial.
Transport at room temperature.
Note: If the sample is NOT kept at 37° C during all processing steps, the cryoprecipitate if present can precipitate out of solution and be lost in the clot or during the centrifugation process and result in a false negative result.
Transport screw-capped vial at room temperature.
Do not refrigerate or freeze.
Note: No tests can be added or poured off this tube; cryocrit and cryoglobulin evaluation only
Allow serum sample to clot for one (1) hour in a 37° C water bath, oven, incubator, or heat block. After clotting, centrifuge the specimen for 10 minutes at 1600 RCF(G) utilizing one of the 3 options below:
1) In a "warm" centrifuge at 37° C.
2) In a bench-top centrifuge placed in a "warm room" at 37° C.
3) At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 1600 RCF(G).
After centrifuging, decant serum into a screw-cap vial.
Transport at room temperature.
Note: If the sample is NOT kept at 37° C during all processing steps, the cryoprecipitate if present can precipitate out of solution and be lost in the clot or during the centrifugation process and result in a false negative result.
Transport screw-capped vial at room temperature.
Do not refrigerate or freeze.
Note: No tests can be added or poured off this tube; cryocrit and cryoglobulin evaluation only
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 72 hours
Refrigerated: Unstable
Frozen: Unstable
Refrigerated: Unstable
Frozen: Unstable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Received refrigerated • Received frozen • Icteric samples • Serum separator tube (SST)
Methodology
Visual Inspection
Setup Schedule
Monday-Sunday Evening
Report available: 4 Days
Report available: 4 Days
Reference Range
None detected
Clinical Significance
Cryoglobulins are a group of proteins which precipitate from serum, plasma or urine upon exposure to cold.
