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HIV-1 RNA, Quantitative, Real-Time PCR with Reflex to Genotype (RTI, PI, Integrase)
Test Code91691
CPT Codes
87536
Includes
If HIV-1 RNA, Quantitative, Real-Time PCR viral load is >400 copies/mL, then HIV-1 Genotype and HIV-1 Integrase Genotype will be performed at an additional charge (CPT code(s): 87900, 87901, 87906).
Preferred Specimen
7 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
3.7 mL
Other Acceptable Specimens
Plasma collected in: PPT Potassium EDTA (white-top) tube
Instructions
Samples submitted in primary tube will be rejected.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube. Follow manufacturer's instructions for collection tube handling.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube. Follow manufacturer's instructions for collection tube handling.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 30 days
Refrigerated: 6 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Samples received in primary collection tube • Gross hemolysis • Lipemia
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Set up: Mon-Sat; Report available: 2-4 days
Reference Range
| HIV-1 RNA, QN, PCR | Not detected | copies/mL | |
| HIV-1 RNA, QN, PCR | Not detected | Log copies/mL | |
Clinical Significance
Monitor viral load in HIV-1 patients undergoing antiretroviral therapy and automatically reflex to drug resistance testing for PR, RT and Integrase drugs in patients failing to achieve or maintain virologic suppression.
