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ANA by IFA, Synovial Fluid (RDL)
Test Code93964
CPT Codes
86038
Includes
If ANA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).
Preferred Specimen
1 mL synovial fluid collected in a sterile container
Minimum Volume
0.3 mL
Instructions
Note: This volume does not allow for repeat testing
Transport Temperature
Refrigerate (cold packs)
Specimen Stability
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 60 days
Refrigerated: 14 days
Frozen: 60 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Bacterial contamination • Lipemia • Icteric • Non-synovial body fluids
Methodology
Immunofluorescence Assay
Setup Schedule
Set up: Daily; Report available: 7-14 days
Reference Range
Negative
Homogeneous Pattern: < 1:40
Nucleolar Pattern: < 1:40
Speckled Pattern: < 1:40
Homogeneous Pattern: < 1:40
Nucleolar Pattern: < 1:40
Speckled Pattern: < 1:40
Clinical Significance
ANA are commonly found in a variety of autoimmune diseases. Antibody frequency increases with age in apparently healthy people. ANA patterns on Hep-2 slides provide only general clues about particles (chromatin, nucleosomes, and spliceosomes). ANA patterns (other than centromere pattern) are not reliably correlated with the presence of specific antibodies and must be further evaluated by EIA using individual ENA antigens.