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Cortisol Response to ACTH Stimulation, Serum
Test Code38149
CPT Codes
82533 (x4)
Includes
Cortisol Response, Baseline
Cortisol Response, 15 Minutes
Cortisol Response, 30 Minutes
Cortisol Response, 60 Minutes
Cortisol Response, 15 Minutes
Cortisol Response, 30 Minutes
Cortisol Response, 60 Minutes
Preferred Specimen
1 mL serum (x4) collected in red-top tubes (no gel)
Minimum Volume
0.2 mL for each timed specimen
Other Acceptable Specimens
Plasma collected in EDTA (lavender-top) tubes
Instructions
Draw morning baseline specimen. Administer 250 mcg Cortrosyn. Draw additional specimens at 15, 30 and 60 minutes.
For each timed specimen submitted, each tube must be clearly marked with time drawn. Submit all tubes with one test request form.
Note: Cortrosyn (cosyntropin) not supplied by the laboratory.
This test cannot be collected at a Patient Service Center.
For each timed specimen submitted, each tube must be clearly marked with time drawn. Submit all tubes with one test request form.
Note: Cortrosyn (cosyntropin) not supplied by the laboratory.
This test cannot be collected at a Patient Service Center.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 14 days
Refrigerated: 21 days
Frozen: 1 year
Refrigerated: 21 days
Frozen: 1 year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Serum separator tubes (SST)
Methodology
Chromatography/Mass Spectrometry
Setup Schedule
Set up: Mon-Sat; Report available: 2-6 days
Reference Range
| Cortisol Baseline | 5-21 mcg/dL |
| Cortisol 15 minutes | 13-30 mcg/dL |
| Cortisol 30 minutes | 14-36 mcg/dL |
| Cortisol 60 minutes | 14-41 mcg/dL |
Clinical Significance
The cortisol response to infusion of synthetic ACTH is useful in diagnosing patients with adrenal insufficiency (Addison's disease).
