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HLA-DRB3,4, 5, Intermediate Resolution, Transplantation
Test Code92161
CPT Codes
81376
Preferred Specimen
14 mL whole blood collected in an EDTA (lavender-top) tube
Pediatric Volumes:
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
> 10 years: 14 mL
Pediatric Volumes:
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
> 10 years: 14 mL
Minimum Volume
5 mL
Other Acceptable Specimens
Whole blood collected in an: acid citrate dextrose ACD (yellow-top), or sodium or lithium heparin (green-top) tube • Minimum: 4 buccal swab
Instructions
Samples cannot be shipped on Saturday or the day before a holiday.
Other Acceptable sample types: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact Versiti Wisconsin for collection, transport and stability instructions.
For Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in EDTA (lavender-top) tubes is required.
For Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Other Acceptable sample types: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact Versiti Wisconsin for collection, transport and stability instructions.
For Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in EDTA (lavender-top) tubes is required.
For Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Transport Temperature
Room temperature
Specimen Stability
Whole blood (preferred)
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swab
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swab
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature >14 days • Received refrigerated or frozen • Hemolysis
Methodology
PCR followed by Sequence Specific Oligonucleotide Probes
Setup Schedule
Set up: Daily; Report available: 7-9 days
Reference Range
See Laboratory Report
