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HLA-A*02:01 Determination
Test Code92044
CPT Codes
81381
Includes
Samples positive for A*0201 require HLA A-High resolution testing for an additional fee.
Preferred Specimen
14 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
5 mL
Other Acceptable Specimens
Whole blood collected in: ACD (yellow-top), sodium or lithium (green-top) tube • Minimum: 4 buccal swabs
Instructions
Sample cannot be shipped on Saturday or the day before a holiday.
Other acceptable sample types: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact Versiti Wisconsin for collection, transport and stability instructions.
Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.
Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Pediatric volume
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL
Other acceptable sample types: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact Versiti Wisconsin for collection, transport and stability instructions.
Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.
Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Pediatric volume
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL
Transport Temperature
Room temperature
Specimen Stability
Whole blood (preferred)
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swab
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swab
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Received room temperature >14 days • Received refrigerated • Received frozen
Methodology
Polymerase Chain Reaction • Direct DNA Sequencing
Setup Schedule
Set up: Mon-Sat; Report available: 7-9 days
Reference Range
See Laboratory Report
