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HPV mRNA E6/E7, Rectal
Test Code91932
CPT Codes
87624
Preferred Specimen
Anal/rectal specimen collected using a dacron/polyester swab or anal/rectal brush and submitted in 3 mL Preservcyt® transport medium ThinPrep® vial or Aptima® specimen transfer tube (green label)
Minimum Volume
1.5 mL
Instructions
To collect an anal-rectal sample, a wetted, non-lubricated Dacron/Polyester swab is used. The Dacron/Polyester swab is inserted about 3 cm (or until resistance is met) into the anal canal past the anal verge, into the rectal vault. This is done without visualization of the anal canal. Firm lateral pressure is applied to the swab handle as it is rotated and slowly moved in and out. Slowly withdraw swab from the anal canal. Swish the swab vigorously in PreservCyt® fluid in the ThinPrep® vial. Discard the swab. Cap and tighten the ThinPrep® vial.
Follow the same procedure if using a brush to collect an anal-rectal sample. Avoid using cotton swab on a wooden stick because the handle may break and splinter during collection. Swab that is grossly contaminated with feces should be discarded and the collection repeated.
Follow the same procedure if using a brush to collect an anal-rectal sample. Avoid using cotton swab on a wooden stick because the handle may break and splinter during collection. Swab that is grossly contaminated with feces should be discarded and the collection repeated.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 30 days
Refrigerated: 30 days
Frozen: Not established
Refrigerated: 30 days
Frozen: Not established
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Digene® vials • SurePath™ vials
Methodology
Transcription-Mediated Amplification (TMA)
Setup Schedule
Set up: Tues, Thurs, Sat; Report available: 1-3 days
Reference Range
Not detected
Clinical Significance
The HPV mRNA E6/E7 test is used in conjunction with cytology to evaluate anal dysplasia.
