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Human Transforming Growth Factor beta 1 (TGF-b1)
Test Code91238
CPT Codes
83520
Preferred Specimen
1 mL plasma collected in a EDTA (lavender-top) tube
Minimum Volume
0.25 mL
Instructions
Collect in EDTA (lavender top) tube. Centrifuge for 15 minutes at 1000 X g within 30 minutes of collection. Centrifuge plasma again at 3000 X g for 10 minutes for complete platelet removal. Freeze at -20°C.
For fixed speed centrifuges such as 645e: Collect in a lavender top tube using EDTA as an anticoagulant. Centrifuge 3 times for 10 minutes at 1600 x g while decanting the plasma each time before the next spin within 30 minutes of collection. Freeze at -20°C or below.
PSC Collections: Collect lavender tube and immediately centrifuge to prepare platelet-poor plasma. Decant the plasma into another pour-over tube. Centrifuge a second time and pour over plasma. Centrifuge a third time and pour over plasma. Freeze immediately.
For fixed speed centrifuges such as 645e: Collect in a lavender top tube using EDTA as an anticoagulant. Centrifuge 3 times for 10 minutes at 1600 x g while decanting the plasma each time before the next spin within 30 minutes of collection. Freeze at -20°C or below.
PSC Collections: Collect lavender tube and immediately centrifuge to prepare platelet-poor plasma. Decant the plasma into another pour-over tube. Centrifuge a second time and pour over plasma. Centrifuge a third time and pour over plasma. Freeze immediately.
Transport Container
Plastic screw-cap vial
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen -20° C: 14 days
Frozen -70° C: 30 days
Refrigerated: 48 hours
Frozen -20° C: 14 days
Frozen -70° C: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed specimens • Grossly lipemic specimens • Specimens with particulate matter or microbial contamination • Specimens outside of listed stability
Methodology
Enzyme Linked Immunosorbent Assay (ELISA)
FDA Status
The performance characteristics of the listed assay was validated by BioAgilytix Diagnostics. The US FDA has not approved or cleared this test. The results of this assay can be used for clinical diagnosis without FDA approval. BioAgilytix Diagnostics is a CLIA certified, CAP accredited laboratory for performing high complexity assays such as this one.
Setup Schedule
Set up: Varies; Report available: 4 business days
Reference Range
344-2382 pg/mL
Clinical Significance
Transforming Growth Factor (TGF) plays a crucial role in tissue regeneration, cell differentiation, embryonic development, and regulation of the immune system. Transforming growth factor beta is found in hematopoietic (blood-forming) tissue and initiates a signaling pathway that suppresses the early development of cancer cells. It enhances the deposition of extracellular matrix and may play potential role in wound healing and cirrhosis formation. Many cells synthesize TGF-b and almost all of them have specific receptors for this peptide.
