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FISH, Locus-specific Probe (x2)
Test Code14656
CPT Codes
88271 (x2), 88275
Includes
This test code is for ordering interphase fluorescence in situ hybridization (FISH) analysis of 100-300 cells using two probes. The locus for the probes must be identified with the order. Please call laboratory before sample submission for availability of probes.
This test is performed in addition to G-banding chromosome analysis. Submit appropriate specimen as required for conventional cytogenetic analysis (amniotic fluid, whole blood, bone marrow, or tissue).
If one or more FISH tests are ordered on a specimen without chromosome analysis, a tissue culture test code will be added at an additional charge.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
This test is performed in addition to G-banding chromosome analysis. Submit appropriate specimen as required for conventional cytogenetic analysis (amniotic fluid, whole blood, bone marrow, or tissue).
If one or more FISH tests are ordered on a specimen without chromosome analysis, a tissue culture test code will be added at an additional charge.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Preferred Specimen
5 mL whole blood collected in a sodium heparin (green-top or royal blue-top or tan-top) tube or
3 mL bone marrow collected in a sodium heparin (green-top or royal blue-top or tan-top) tube or
5 mL amniotic fluid collected in a sterile container or
5 mg CVS collected in culture media or
5x5 mm fresh tissue collected in a sterile container or Formalin Fixed Paraffin Embedded Tissue
3 mL bone marrow collected in a sodium heparin (green-top or royal blue-top or tan-top) tube or
5 mL amniotic fluid collected in a sterile container or
5 mg CVS collected in culture media or
5x5 mm fresh tissue collected in a sterile container or Formalin Fixed Paraffin Embedded Tissue
Minimum Volume
1 mL whole blood • 1 mL bone marrow • 5 mL amniotic fluid • 5 mg CVS
Instructions
Clincal history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.
Specimen requirements: 3-5 mL Whole blood or bone marrow collected in sodium heparin tube, 5 mL Amniotic fluid collected in sterile container, 5 mg CVS in culture media, 5X5 mm Fresh tissue in sterile container, Formalin Fixed Paraffin Embedded Tissue.
Specimen viability decreases during transit. Send specimen to testing laboratory for viability determination. Do not freeze. Do not reject.
Specimen requirements: 3-5 mL Whole blood or bone marrow collected in sodium heparin tube, 5 mL Amniotic fluid collected in sterile container, 5 mg CVS in culture media, 5X5 mm Fresh tissue in sterile container, Formalin Fixed Paraffin Embedded Tissue.
Specimen viability decreases during transit. Send specimen to testing laboratory for viability determination. Do not freeze. Do not reject.
Transport Container
See preferred specimen requirements
Transport Temperature
Room temperature
Specimen Stability
Room temperature: See instructions
Refrigerated: See instructions
Frozen: See instructions
Refrigerated: See instructions
Frozen: See instructions
Methodology
Fluorescence in situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 5 days
Reference Range
See Laboratory Report
Clinical Significance
This FISH (fluorescence in situ hybridization) assay is performed using any combination of two probes.
