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Francisella tularensis Ab (IgG,IgM)
Test Code18516
Preferred Specimen
Collection Instruction:
Centrifuge and aliquot serum into a plastic vial.
Reject Criteria:
Gross hemolysi, grossly lipemic, grossly icteric
PREFERRED
0.6 mL serum (SS, serum separator tube) or (R, red-top
tube (no gel), room temperature
Minimum: 0.5 mL
RT: 7 days
Refrigerated (cold packs): 14 days
Frozen: 30 days
Centrifuge and aliquot serum into a plastic vial.
Reject Criteria:
Gross hemolysi, grossly lipemic, grossly icteric
PREFERRED
0.6 mL serum (SS, serum separator tube) or (R, red-top
tube (no gel), room temperature
Minimum: 0.5 mL
RT: 7 days
Refrigerated (cold packs): 14 days
Frozen: 30 days
Minimum Volume
0.5 mL
Transport Container
Plastic Transport Vial
Transport Temperature
Room Temperature
Specimen Stability
Room Temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Methodology
Enzyme-Linked Immunosorbent Assay Immunoassay
Setup Schedule
Tuesday, Thursday, Saturday Morning
Report available: 4 Days
Report available: 4 Days
Reference Range
F. tularensis Ab (IgG) Negative
F. tularensis Ab (IgM) Negative
F. tularensis Ab (IgM) Negative
Clinical Significance
This immunoassay is for the qualitative detection of
antibodies (IgG, IgM) against Francisella tularensis, the
causative agent of Tularemia disease. Antibodies can be
detected starting 2-3 weeks after the onset of symptoms.
Paired sera 2-3 weeks apart to observe seroconversion is
recommended to confirm infection in patients with high
clinical suspicion.
This assay is intended for patients with signs and
symptoms consistent with acute Tularemia and not useful
for patients with resolved infection. Tularemia can cause
a wide spectrum of symptoms depending on exposure. People
rarely can be infected through tick and deer fly bites,
contact with infected animals, drinking contaminated
water, or inhalation.
Antibodies against other bacteria, including Brucella,
can cross-react with assays targeting Francisella.
Therefore, positive serologic results should be
interpreted in the context of clinical illness and
exposure consistent with Tularemia infection. Antibodies
can persist for years and IgM in a single sample is not a
reliable indicator of recent infection. Positive IgG and
IgM results can represent current, recent, or past
infection since they can be detected for several years
post infection. A
negative result can occur early during acute infection,
before the development of antibodies.
References:
1. Clinical Testing and Diagnosis for Tularemia,
Centers for Disease Control and Prevention.
https://www.cdc.gov/tularemia/hcp/diagnosis-testing/index.h
tml Last updated May 15, 2024.
2. Jeannine M Petersen, and Elizabeth A Dietrich,
2023. 13.4 Detection of Francisella tularensis
Antibodies, Leber AL, Burnham CD Clinical Microbiology
Procedures Handbook, 5th Edition. ASM Press, Washington,
DC. doi:
10.1128/9781683670438.CMPH.ch13.4.
3. Maurin, Max. Francisella tularensis, Tularemia and
Serological Diagnosis. Front Cell Infect Microbiol. 2020;
10:512090.
antibodies (IgG, IgM) against Francisella tularensis, the
causative agent of Tularemia disease. Antibodies can be
detected starting 2-3 weeks after the onset of symptoms.
Paired sera 2-3 weeks apart to observe seroconversion is
recommended to confirm infection in patients with high
clinical suspicion.
This assay is intended for patients with signs and
symptoms consistent with acute Tularemia and not useful
for patients with resolved infection. Tularemia can cause
a wide spectrum of symptoms depending on exposure. People
rarely can be infected through tick and deer fly bites,
contact with infected animals, drinking contaminated
water, or inhalation.
Antibodies against other bacteria, including Brucella,
can cross-react with assays targeting Francisella.
Therefore, positive serologic results should be
interpreted in the context of clinical illness and
exposure consistent with Tularemia infection. Antibodies
can persist for years and IgM in a single sample is not a
reliable indicator of recent infection. Positive IgG and
IgM results can represent current, recent, or past
infection since they can be detected for several years
post infection. A
negative result can occur early during acute infection,
before the development of antibodies.
References:
1. Clinical Testing and Diagnosis for Tularemia,
Centers for Disease Control and Prevention.
https://www.cdc.gov/tularemia/hcp/diagnosis-testing/index.h
tml Last updated May 15, 2024.
2. Jeannine M Petersen, and Elizabeth A Dietrich,
2023. 13.4 Detection of Francisella tularensis
Antibodies, Leber AL, Burnham CD Clinical Microbiology
Procedures Handbook, 5th Edition. ASM Press, Washington,
DC. doi:
10.1128/9781683670438.CMPH.ch13.4.
3. Maurin, Max. Francisella tularensis, Tularemia and
Serological Diagnosis. Front Cell Infect Microbiol. 2020;
10:512090.
