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Influenza A and B RNA, QL RTPCR,Conjunct w/rfl H5
Test Code18203
Includes
If Influenza A RNA, PCR is detected, then Influenza A Virus RNA, H5 Subtyping, Qualitative Real-Time PCR will be performed at an additional charge (CPT code(s): 87503).
Preferred Specimen
**This test is not available for New York patient testing**
Collection Instructions:
Each Influenza type A/B RNA with reflex to H5 specimen
should be accompanied by its own separate requisition and
transported in its own sealed bag. If swabbing both eyes,
it is acceptable to place both swabs into one vial of VCM
or equivalent. This test is not approved for observed-self
collection at Quest Patient Service Centers.
Reject Criteria:
Calcium alginate swabs; Wooden-shafted swabs; Nasal
specimens
PREFERRED
1 or 2 conjunctiva swabs submitted in 3 mL (0.6 mL minimum)
VCM (green-cap) or equivalent (UTM) tube, refrigerated
(cold packs)
RT: Unacceptable
Refrigerated (cold packs): 4 days
Frozen -20 degrees C: Unacceptable
Frozen -70 degrees C: 30 days
Collection Instructions:
Each Influenza type A/B RNA with reflex to H5 specimen
should be accompanied by its own separate requisition and
transported in its own sealed bag. If swabbing both eyes,
it is acceptable to place both swabs into one vial of VCM
or equivalent. This test is not approved for observed-self
collection at Quest Patient Service Centers.
Reject Criteria:
Calcium alginate swabs; Wooden-shafted swabs; Nasal
specimens
PREFERRED
1 or 2 conjunctiva swabs submitted in 3 mL (0.6 mL minimum)
VCM (green-cap) or equivalent (UTM) tube, refrigerated
(cold packs)
RT: Unacceptable
Refrigerated (cold packs): 4 days
Frozen -20 degrees C: Unacceptable
Frozen -70 degrees C: 30 days
Methodology
Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Monday-Sunday All Shifts
Report available: 2 Days
Report available: 2 Days
Reference Range
| Influenza A RNA, PCR | Not Detected |
| Influenza B RNA, PCR | Not Detected |
| Influenza A Virus RNA, H5 Subtyping, QL RT PCR | Not Detected |
Clinical Significance
This test is for detection of Influenza A virus, with reflex to H5 typing. The H5 reflex is a qualitative molecular diagnostic test performed on Influenza A positive samples to identify Influenza A H5 virus, including H5N1, commonly known as Avian Influenza. The reflex test does not subtype for seasonal influenza viruses. This test is intended to be performed on conjunctiva specimens collected from individuals suspected of infection with Influenza A H5, or who meet the Centers for Disease Control and Prevention (CDC) clinical and/or epidemiological criteria for Influenza A H5N1 testing. For details visit: https://www.cdc.gov/bird-flu/hcp/clinicians-evaluating-patients.
Laboratory test results should always be considered in the context of clinical observations and epidemiological data in making a final diagnosis and patient management decisions.
Laboratory test results should always be considered in the context of clinical observations and epidemiological data in making a final diagnosis and patient management decisions.
