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Influenza A and B RNA, QL RT PCR Resp w/refl H5
Test Code18201
Includes
If Influenza A RNA, PCR is Detected, then Influenza A Virus RNA, H5 Subtyping, Qualitative Real-Time PCR will be performed at an additional charge (CPT code(s): 87503).
Preferred Specimen
**This test is not available for New York patient testing**
Collection Instructions:
Each Influenza Type A/B RNA with Reflex to H5 specimen
should be accompanied by its own separate requisition and
transported in its own sealed bag. This test is not
approved for observed-self collection at Quest Patient
Service Centers.
Reject Criteria:
Calcium alginate swabs; Wooden-shafted swabs; Nasal
specimens
PREFERRED
Nasopharyngeal swab or anterior nares or oropharyngeal swab
submitted in 3 mL (0.6 mL minimum) VCM (green-cap) or
equivalent (UTM) tube, refrigerated (cold packs)
Minimum: 1 swab
--or--
ACCEPTABLE
1 mL bronchial lavage/wash collected in a sterile,
leakproof container, refrigerated (cold packs)
Minimum: 1 mL
RT: Unacceptable
Refrigerated (cold packs): 4 days
Frozen -20 degrees C: Unacceptable
Frozen -70 degrees C: 30 days
Collection Instructions:
Each Influenza Type A/B RNA with Reflex to H5 specimen
should be accompanied by its own separate requisition and
transported in its own sealed bag. This test is not
approved for observed-self collection at Quest Patient
Service Centers.
Reject Criteria:
Calcium alginate swabs; Wooden-shafted swabs; Nasal
specimens
PREFERRED
Nasopharyngeal swab or anterior nares or oropharyngeal swab
submitted in 3 mL (0.6 mL minimum) VCM (green-cap) or
equivalent (UTM) tube, refrigerated (cold packs)
Minimum: 1 swab
--or--
ACCEPTABLE
1 mL bronchial lavage/wash collected in a sterile,
leakproof container, refrigerated (cold packs)
Minimum: 1 mL
RT: Unacceptable
Refrigerated (cold packs): 4 days
Frozen -20 degrees C: Unacceptable
Frozen -70 degrees C: 30 days
Methodology
Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Monday-Sunday All Shifts
Report available: 2 Days
Report available: 2 Days
Reference Range
| Influenza A RNA, PCR | Not Detected |
| Influenza B RNA, PCR | Not Detected |
| Influenza A Virus RNA, H5 Subtyping, QL RT PCR | Not Detected |
Clinical Significance
This screening test reflexes to the Influenza A (H5) Virus RNA, Qualitative Real-Time PCR test when influenza A is detected. The H5 reflex is a qualitative multi-target molecular diagnostic test that aids in the detection of the Influenza A H5 virus, including H5N1, commonly known as Avian Influenza. The reflex test does not detect Influenza B or other respiratory viurses. This test is intended to be performed on respiratory specimens collected from individuals suspected of infection with Influenza A H5, or who meet the Centers for Disease Control and Prevention (CDC) clinical and/or epidemiological criteria for Influenza A H5N1 testing. For details visit: https://www.cdc.gov/bird-flu/hcp/clinicians-evaluating-patients.
Laboratory test results should always be considered in the context of clinical observations and epidemiological data in making a final diagnosis and patient management decisions.
Laboratory test results should always be considered in the context of clinical observations and epidemiological data in making a final diagnosis and patient management decisions.
