C4a Level by RIA

Test Code

14072

CPT Codes
86160<br>Restricted Client Code

Preferred Specimen

1 mL frozen plasma collected in a K2 EDTA (lavender-top) tube

Pediatric volume: 0.5 mL minimum

Minimum Volume

0.5 mL

Instructions

Plasma from a lavender top (K2- EDTA) tube is the only acceptable sample type. The EDTA collection tube must be completely filled with blood to ensure the correct blood to anticoagulant ratio. Invert tube several times for thorough mixing of blood anticoagulants. Keep the EDTA tube filled with blood on ice until centrifugation. Within 30 (+/-5) minutes of venipuncture, centrifuge the collection tube at 1600 (+/- 200) x g (rcf) at 4°C (+/- 2°C) for 15 (+/-1) minutes. Transfer the cell-free plasma to a clean cryogenic storage tube and immediately store samples at -20°C, or freeze at -60°C or below in an ultra-low freezer.

Ambient temperature for centrifugation is acceptable provided that the time between collection and storage is minimized (approximately 30 minutes). Ship samples overnight in a well-insulated container on dry ice.

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 28 days
Frozen -70° C: 2 years

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received thawed • Samples stored at room temperature prior to shipment

Methodology
RadioImmunoassay (RIA)

Setup Schedule

Set up: Mon-Fri; Report available: 7-10 days

Reference Range

0-2830 ng/mL

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.