Mycobacteria,Cult,w/Smear Non Sputum (REFL)

Test Code

14274

CPT Codes
87015, 87116, 87206<br>Restricted Client Code

Includes

Mycobacteria Culture, Concentration, Acid-Fast Bacilli Stain:
An additional charge for Concentration (CPT code(s): 87015) will be added as appropriate.

If Acid Fast bacilli is seen on smear, Mycobacterium tuberculosis Complex and Rifampin Resistance will be performed at an additional charge (CPT code(s): 87801).

Identification will be performed at an additional charge for each organism: CPT code(s) 87118:

Matrix Assisted Laser Desorption/Ionization-Time of Flight:
If the organism cannot be identified by the original method additional charges will be added for each organism:
CPT code(s) 87153: AFB Sequencing.

If TB is isolated for the first time or after three months after the first isolate from a patient, susceptibilities
will be performed at an additional charge (CPT code(s): 87190 per drug).

If other Mycobacteria species are isolated, appropriate susceptibilities can be performed at an additional charge
if requested (CPT code(s): 87186 per organism).

Preferred Specimen

10 mL body fluids or lower respiratory tract specimens, bronchial brush/wash, or
3 mL CSF, or
2 grams fresh (unfixed) tissue, or
40 mL urine

Patient Preparation
Expectorated sputum: Instruct patient to gargle with water and cough deeply. Three separate early morning collections on at least three consecutive days are recommended.

Minimum Volume

3 mL body fluids • 1.5 mL CSF • 1 gram fresh tissue • 20 mL urine

Instructions

Tissue: Sterile leak-proof container with small amount of sterile, non-bacteriostatic saline (no fixative or preservative).

Note: See the alphabetical listing in the Microbiology section of specimen Collection and Handling for further instructions.

Transport Container

Sterile leak-proof container

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 5 days
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

24-hour pooled urine • Dry swabs • Specimens received in alcohol, formalin, EDTA, lithium heparin or conventional blood culture bottles • Throat/oral swabs from non-cystic-fibrosis patients • Urine specimens in preservative tubes • Processed/concentrated specimen from client

Methodology
Continuous Monitoring Broth Culture System • Conventional Culture • Microscopic Exam with Fluorochrome Stain • Real-Time Polymerase Chain Reaction (RT-PCR)

Setup Schedule

Set up: Mon-Sat; Report available: 6-8 weeks

Reference Range

See Laboratory Report

Clinical Significance

There are more than 70 species of mycobacteria of which the primary pathogen is M. tuberculosis. Tuberculosis is a chronic,
granulomatous disease caused by M. tuberculosis. Identification, and, if appropriate, antibiotic sensitivity, is required to initiate necessary therapy.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.