HSV, Type 1 and 2 DNA, QN Real Time PCR, Serum

Test Code

16584

CPT Codes
87530 (x2)<br>Restricted Client Code

Preferred Specimen

1 mL CSF, vitreous, pericardial, pleural, or amniotic fluid collected in a sterile leak-proof container, or
1 mL frozen serum, or
1 mL plasma or whole blood collected in an EDTA (lavender-top) tube, or ACD (yellow-top) tube

Minimum Volume

0.3 mL

Other Acceptable Specimens

Bronchoalveolar lavage or bronchial wash collected in a sterile leak-proof container

Transport Container

Transport tube

Transport Temperature

CSF, vitreous, pericardial, pleural, or amniotic fluid: Frozen
Serum and plasma: Frozen
BAL or bronchial wash: Frozen
Whole blood: Refrigerated (cold packs)

Specimen Stability

Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days

Methodology
Real-Time PCR

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Daily; Report available: 1 day

Reference Range

HSV-1 DNA, QN PCR: Not detected
HSV-2 DNA, QN PCR: Not detected

Clinical Significance

The detection of HSV-1 and HSV-2 DNA is based upon the real-time amplification, detection and differentiation of specific HSV-1 and HSV-2 genomic DNA sequences by PCR from total DNA extracted from the specimen. The quantitative range of this assay is 100 - 2,000,000 copies/mL.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.