Oropouche Virus RNA, Qualitative Real-Time Reverse Transcriptase (RT) PCR

Test Code

14328

CPT Codes
87798

Preferred Specimen

1.5 mL serum or
1.5 mL CSF collected in a plastic sterile leak-proof container

Minimum Volume

0.5 mL

Transport Container

Plastic sterile leak-proof container

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Samples using heparin as anticoagulant

Methodology
Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)

Setup Schedule

Set up: Daily; Report available: 2-3 days

Reference Range

Not Detected

Clinical Significance

This test is intended for the detection of Oropouche virus (OROV) RNA in serum or CSF from patients with exposure, signs or symptoms consistent with OROV infection. It can be used to help in the diagnosis of Oropouche fever infection during the acute phase of the disease, which is typically within 7 days post-symptom onset. Serological testing can be considered for confirmatory diagnosis during the convalescent phase of this disease (greater than 7 days). This molecular test cannot distinguish between closely related reassortant OROVs within the Orthobunyavirus genus including Madre de Dios Virus, Iquitos Virus and Perdoes Virus. The results of this test should be interpreted in the context of pertinent clinical findings and history.

Reference
1. Wesselmann K, et al. Lancet infectious Diseases. 2024;24 :e439-452

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.