|
|
| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Vitamin B1 (Thiamine), Plasma (Lavender, Room temp, Light protected)
Test Code14159
CPT Codes
84425<br>Client Restricted Code
Preferred Specimen
2 mL plasma collected from an EDTA (lavender-top) tube - protected from light
Patient Preparation
Overnight fasting is required
Minimum Volume
1 mL
Other Acceptable Specimens
Plasma submitted in a foil wrapped tube - protected from light • Serum submitted in a amber transport tube or foil wrapped tube - protected from light
Instructions
Collect blood in a lavender-top tube. Remove plasma within 4 hours of collection and transfer to a light protected pour off tube. The separation of cells must be completed within 24 hours of collection. The separation of cells must be completed within 4 hours of collection. Separate cells by centrifugation at 2-8° C for 8-10 minutes. Transfer plasma to amber tube to protect from light and freeze tube immediately. Alternately, neutral-colored tubes can be used if wrapped in aluminum foil. Ship frozen tubes at -10 to -30° C.
Serum (acceptable): Collect blood in plain red-top evacuated tube. Allow to clot at 20-25° C for 20-30 minutes. Centrifuge at 2-8° C for 8-10 minutes. Transfer serum to an amber polypropylene or polyethylene transport tube to protect from light and freeze tube immediately. Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil. Ship Frozen tubes at -10 to -30° C.
Serum (acceptable): Collect blood in plain red-top evacuated tube. Allow to clot at 20-25° C for 20-30 minutes. Centrifuge at 2-8° C for 8-10 minutes. Transfer serum to an amber polypropylene or polyethylene transport tube to protect from light and freeze tube immediately. Alternately, neutral colored polypropylene or polyethylene tubes can be used if wrapped in aluminum foil. Ship Frozen tubes at -10 to -30° C.
Transport Container
Amber tube - protected from light
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 48 hours
Frozen: 30 days
Refrigerated: 48 hours
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Lipemia • Received not protected from light
Methodology
Chromatography/Mass Spectrometry
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Tues-Sat; Report available: 3-5 days
Reference Range
See Laboratory Report
Clinical Significance
Vitamin B1 deficiency is most often associated with alcoholism, chronic illness and following gastric by-pass surgery. Prolonged deficiency causes beriberi. Plasma vitamin B1 is useful in evaluating nutritional assessment and compliance, while whole blood vitamin B1 is useful in evaluating body stores.
