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C3a Level By RIA, Plasma
Test Code13526
CPT Codes
86160<br>Restricted Client Code
Preferred Specimen
1 mL plasma submitted in a sterile, plastic screw cap vial
Minimum Volume
0.5 mL
Instructions
Plasma from a lavender top (EDTA) tube is the only acceptable sample type. Mix sample thoroughly. Keep tubes cold on wet ice or with a cold pack after collection.
Within 30 minutes of venipuncture, centrifuge at 4°C. Transfer the cell-free plasma to a clean tube and immediately freeze on dry ice or at -70°C or below.
Ambient temperature for centrifuging is acceptable provided that the time between collection, spinning and decanting to the freezer, is minimized (approximately 30 minutes).
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 1 year
Refrigerated: Unacceptable
Frozen: 1 year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Thawed specimen
Methodology
Radial Immunodiffusion
Setup Schedule
Set up: Varies; Report available: 21 days
Reference Range
See Laboratory Report