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| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Iron, Liver Tissue
Test Code13243
CPT Codes
83540
Preferred Specimen
2 cm liver tissue collected with a 22-gauge needle or
1 cm liver tissue collected with an 18-gauge needle submitted in a trace metal-free (royal blue-top) tube
1 cm liver tissue collected with an 18-gauge needle submitted in a trace metal-free (royal blue-top) tube
Minimum Volume
2 mg
Other Acceptable Specimens
2 mg tissue in paraffin block (Note: if no more than 1 or 2 cuts have been made to it for slides)
Instructions
Necessary information: Patient's date of birth is required to calculate iron index.
1. Two mg of liver tissue is required. This is typically a piece of tissue from a 22-gauge needle biopsy at least 2 cm long. If an 18-gauge needle is used, the tissue must be at least 1 cm in length.
2. Any specimen vial other than a Mayo metal-free vial used should be plastic, leached with 10% nitric acid for 2 days, rinsed with redistilled water, and dried in clean air.
Additional information: Paraffin blocks will be returned 3 days after analysis is complete.
Cautions:
Formalin-fixed, paraffin-embedded tissue can be used when histologic examination (including iron stains) is requested in addition to the hepatic iron concentration and calculated hepatic iron index; however, fresh or frozen tissue is preferred.
Paraffin blocks from which many slides have been previously cut often appear to contain sufficient quantity of tissue, however, the specimen is often very thin and less than 2 mg in weight.
1. Two mg of liver tissue is required. This is typically a piece of tissue from a 22-gauge needle biopsy at least 2 cm long. If an 18-gauge needle is used, the tissue must be at least 1 cm in length.
2. Any specimen vial other than a Mayo metal-free vial used should be plastic, leached with 10% nitric acid for 2 days, rinsed with redistilled water, and dried in clean air.
Additional information: Paraffin blocks will be returned 3 days after analysis is complete.
Cautions:
Formalin-fixed, paraffin-embedded tissue can be used when histologic examination (including iron stains) is requested in addition to the hepatic iron concentration and calculated hepatic iron index; however, fresh or frozen tissue is preferred.
Paraffin blocks from which many slides have been previously cut often appear to contain sufficient quantity of tissue, however, the specimen is often very thin and less than 2 mg in weight.
Transport Container
See Collection Instructions
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Liver tissue
Room temperature: Unacceptable
Refrigerated: 10 days
Frozen: Unacceptable
Tissue paraffin block
Room temperature: Indefinitely
Refrigerated: Indefinitely
Frozen: Indefinitely
Room temperature: Unacceptable
Refrigerated: 10 days
Frozen: Unacceptable
Tissue paraffin block
Room temperature: Indefinitely
Refrigerated: Indefinitely
Frozen: Indefinitely
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability
Methodology
Inductively Coupled Plasma-Mass Spectrometry
FDA Status
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.
Setup Schedule
Set up: Mon, Thurs; Report available: 3-6 days
Reference Range
| Iron Liver Tissue | |
| Male | 400-2400 mcg/g dry wt |
| Female | 200-1800 mcg/g dry wt |
| Hepatic Iron Index | |
| <13 years | Not established |
| ≥13 years | <1.0 mcmol/g/yr |
Clinical Significance
Diagnosis of hemochromatosis using liver tissue specimens.
