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Wet Mount (GL) (Genital Specimens)
MessagePerformed in Microbiology
Microscopy exam will determine the presence of Trichomonas, yeast cells, "Clue cells" and WBCs.
BUMCT: Only Trichomonas reported. Order Gram Stain for evaluation of Bacterial Vaginosis (BV).
Microscopy exam will determine the presence of Trichomonas, yeast cells, "Clue cells" and WBCs.
BUMCT: Only Trichomonas reported. Order Gram Stain for evaluation of Bacterial Vaginosis (BV).
Test Code
WETM
Alias/See Also
Genital Candidiasis
Trichomoniasis
Trichomoniasis
Preferred Specimen
Vaginal discharge collected using culture swab submitted in approximately 2 ml sterile nonbacteriostatic saline
Urethral discharge
Prostatic secretions collected in sterile screw-capped container
Urethral discharge
Prostatic secretions collected in sterile screw-capped container
Minimum Volume
Must contain at least 1ml of fluid
Instructions
Describe specific source, such as:
Vaginal discharge
Urethral discharge
Prostatic secretions
Vaginal discharge
Urethral discharge
Prostatic secretions
Transport Container
Plastic or glass tube with 2 ml of saline
Transport Temperature
Ambient / room temperature
Specimen Stability
For fluid or Swabs: Send to the Lab within 30 minutes of collection.
Transport to the lab at ambient / room temperature
Transport to the lab at ambient / room temperature
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unacceptable specimens:
Specimen types other that those listed
eSwab
Dry swabs (without saline)
Refrigerated specimens
Tubes filled with more than approximately 2 ml of saline
Specimens with delayed transport time >1 hour will be rejected
Specimen types other that those listed
eSwab
Dry swabs (without saline)
Refrigerated specimens
Tubes filled with more than approximately 2 ml of saline
Specimens with delayed transport time >1 hour will be rejected
Methodology
Microscopic examination
Report Available
Reported <2 hours
Limitations
Specimens must be examined within 1 hour of collection to detect motile Trichomonas. If it is not possible to examine specimen within 1 hour, it is recommended to order Trichomonas vaginalis amplified nucleic acid detection. Reference: Sex Transm Infect. 2013 Sep;89(6):485-8.
Clinical Significance
Trichomonas vaginalis, a protozoan that can be carried asymptomatically in the vagina, is significantly associated with symptoms of yellow discharge, abnormal vaginal odor, vulvar itching, and/or colpitis macularis. Carriage in the third trimester is associated with an increased risk of premature rupture of membranes, premature delivery, and low birth weight. Every effort should be made to transport wet mount specimens to the laboratory for testing within 30 minutes of collection. After 30 minutes outside of the body, Trichomonas begin to perish.
Vulvovaginal candidiasis, (VC) accounts for about one-third of the vaginitis cases seen in private practice. Yeast carried vaginally in small numbers and producing no symptoms may be considered as part of normal flora.
Bacterial vaginosis (BV) or nonspecific vaginitis is a common clinical condition characterized by a discharge that is thin, milky, and/or creamy. The etiology of bacterial vaginosis is poorly understood, but is thought to involve a shift in flora from lactobacilli spp. to increased numbers of anaerobes and synergistic mixture of a multitude of microorganisms often illustrated with clue cells. In addition to causing varying degrees of vaginal discomfort, bacterial vaginosis in pregnancy carries an increased risk of adverse outcome, including lower birth weight, intra-amniotic infection, preterm labor, and premature rupture of membranes.
Vulvovaginal candidiasis, (VC) accounts for about one-third of the vaginitis cases seen in private practice. Yeast carried vaginally in small numbers and producing no symptoms may be considered as part of normal flora.
Bacterial vaginosis (BV) or nonspecific vaginitis is a common clinical condition characterized by a discharge that is thin, milky, and/or creamy. The etiology of bacterial vaginosis is poorly understood, but is thought to involve a shift in flora from lactobacilli spp. to increased numbers of anaerobes and synergistic mixture of a multitude of microorganisms often illustrated with clue cells. In addition to causing varying degrees of vaginal discomfort, bacterial vaginosis in pregnancy carries an increased risk of adverse outcome, including lower birth weight, intra-amniotic infection, preterm labor, and premature rupture of membranes.
