Disaccharidases

Message

Non-interface to QNI

Test Code

AP DIDASE

Quest Code

19701

CPT Codes
82657 (x4)

Includes

Lactase, Maltase, Palatinase, Sucrase

Preferred Specimen

5 mg frozen small bowel biopsy collected in a 2 mL cryovial polypropylene screw-cap tube

Minimum Volume

2 mg

Instructions

Place 5 mg small bowel biopsy on the interior surface of a 2 mL polypropylene screw-cap cryovial or small polypropylene collection tube. Freeze within 2 hours of collection at -20° C.

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 31 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Samples frozen later than 2 hours post collection • Samples received in urine containers • Samples immersed in a colorless fluid (i.e., water, formalin, saline, etc.) • Samples placed on gauze, filter paper or any other type of support media

Methodology
Spectrophotometry (SP)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Set up: Sun-Fri; Report available: 1-3 days

Reference Range

Lactase	15.0-45.5 uM/min/g prot
Sucrase	25.0-69.9 uM/min/g prot
Maltase	100.0-224.4 uM/min/g prot
Palatinase	5.0-26.3 uM/min/g prot

Clinical Significance

Lactase, maltase, palatinase and sucrase are mucosal disaccharidases that are present in normal small intestine and fetal colon. A decrease in the enzyme activity of these disaccharidases has been noted in colonic adenomas, adenocarcinomas and malabsorption. Decreased disaccharidase activity is a good indication of mucosal injury with the exception of lactase.

Performing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.