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CK
MessageTest performed at Portsmouth Hospital Laboratory.
Test Code
PORTSCK
Alias/See Also
Creatine Kinase
Creatine Phosphokinase (CPK)
Creatine Phosphokinase (CPK)
CPT Codes
82550
Preferred Specimen
0.2 mL serum collected in a serum separator tube (SST)
Other Acceptable Specimens
0.2 mL serum collected in a no additive (red-top) tube, transferred to a plastic screw-cap vial
Transport Temperature
Refrigerated
Specimen Stability
Room temperature: 4 hours
Refrigerated: 5 days
Frozen: 1 month
Refrigerated: 5 days
Frozen: 1 month
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed specimen
Methodology
Spectrophotometry
Upper Limit: 100,000 U/L
Setup Schedule
Daily
Reference Range
| Male | 39-308 U/L |
| Female | 26-192 U/L |
Clinical Significance
CK is commonly elevated in myocarditis of any cause, cerebrovascular accidents, rhabdomyolysis, polymyositis, and acute physical exertion. CK is also increased in the muscular dystrophies. Low CK may reflect decreased muscle mass or muscle wasting. Low serum CK activities are common in the elderly, in the bedridden, and in patients with advanced malignancy.
From Vista Instrument at Portsmouth:
Intended Use: The CK method is an in vitro diagnostic test for the quantitative measurement of CK in human serum and plasma on the vista system.
Summary: Measurements of CK are used in the diagnosis and treatment of myocardial infarction and muscle diseases. CK may also be elevated following muscle injury or strenuous exercise.
This method for measurement of total CK activity concentration uses the international federation of clinical chemistry (IFCC) CK 37C primary reference method procedure, adapted to the dimension vista system. The dimension vista CK results are standardized to agree with results of the IFCC 37C method.
Principles of Procedure: In a coupled enzyme reaction, the CK in patient samples catalyzes the transphosphorylation of phosphate from creatine phosphate to adenosine diphosphate (ADP) producing adenosine triphosphate (ATP). Hexokinase (HK) phosphorylates glucose from the ATP. The resulting glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase (G-6-PDH) with the simultaneous reduction of nicotinamide adenine dinucleotide phosphate (NADP). The rate of formation of NADHP is directly proportional to the CK activity in the sample and is measured bichromatically at 340 and 540 nm.
From Vista Instrument at Portsmouth:
Intended Use: The CK method is an in vitro diagnostic test for the quantitative measurement of CK in human serum and plasma on the vista system.
Summary: Measurements of CK are used in the diagnosis and treatment of myocardial infarction and muscle diseases. CK may also be elevated following muscle injury or strenuous exercise.
This method for measurement of total CK activity concentration uses the international federation of clinical chemistry (IFCC) CK 37C primary reference method procedure, adapted to the dimension vista system. The dimension vista CK results are standardized to agree with results of the IFCC 37C method.
Principles of Procedure: In a coupled enzyme reaction, the CK in patient samples catalyzes the transphosphorylation of phosphate from creatine phosphate to adenosine diphosphate (ADP) producing adenosine triphosphate (ATP). Hexokinase (HK) phosphorylates glucose from the ATP. The resulting glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase (G-6-PDH) with the simultaneous reduction of nicotinamide adenine dinucleotide phosphate (NADP). The rate of formation of NADHP is directly proportional to the CK activity in the sample and is measured bichromatically at 340 and 540 nm.
Performing Laboratory
Portsmouth Hospital Laboratory.
