Platelet Antibody, Direct

Test Code
PLTD


Quest Code
5019


CPT Codes
86023

Preferred Specimen
Whole blood collected in an EDTA (lavender-top) tube - (see instructions)


Minimum Volume
See Instructions


Instructions
Do not refrigerate or freeze.

Please note: Effective 11/01/96, specimens submitted in ACD tubes (yellow-top) will no longer be accepted because of the high incidence of platelet clumping associated with this anticoagulant. If the platelet count is less than 20,000/ccm, submit 7-10 mL of whole blood.

Platelet Count Minimum Volume:
≥ 45,000 2 mL
20,000-45,000 5 mL
<20,000 10 mL

Pediatric minimum volume: The minimum volume is platelet count dependent. If possible, submit at least 1 mL EDTA blood


Transport Temperature
Room temperature


Specimen Stability
Room temperature: 6 days
Refrigerated: Unacceptable
Frozen: Unacceptable


Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Received frozen • ACD tubes


Methodology
Flow Cytometry (FC)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule
Set up: Daily; Report available: 4-5 days


Reference Range
Anti-IgG Negative


Clinical Significance
Thrombocytopenia that is refractory to platelet transfusions may be due to direct platelet antibody. Testing is useful to differentiate immune from non-immune disorders.


Performing Laboratory
Quest Diagnostics Nichols Institute-San Juan Capistrano, CA
33608 Ortega Highway
San Juan Capistrano, CA 92675-2042




The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.