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M. pneumoniae DNA PCR, Respiratory
Test CodeMYCOPNEU
Alias/See Also
Mycoplasma pneumoniae DNA, Qualitative Real-Time PCR
Quest Code
15498
Quest Code
15498
CPT Codes
87581
Preferred Specimen
1 mL BAL/wash, submitted in a sterile plastic leakproof container
-or-
1 mL sputum, submitted in a sterile plastic leakproof container
-or-
1 mL sputum, submitted in a sterile plastic leakproof container
Minimum Volume
0.3 mL BAL/wash, or sputum
0.35 mL Pericardial fluid, CSF, or pleural fluid
Nasopharyngeal or throat swab in 0.3 mL M4, VCM, or UTM
0.35 mL Pericardial fluid, CSF, or pleural fluid
Nasopharyngeal or throat swab in 0.3 mL M4, VCM, or UTM
Other Acceptable Specimens
Nasopharyngeal or throat swab in 3 mL M4, VCM or UTM Transport Medium
1 mL pericardial or pleural fluid, submitted in a sterile plastic leakproof container
1 mL CSF, submitted in a sterile plastic leakproof container
>3mm3 fresh tissue, submitted in a sterile, plastic leakproof container
1 mL pericardial or pleural fluid, submitted in a sterile plastic leakproof container
1 mL CSF, submitted in a sterile plastic leakproof container
>3mm3 fresh tissue, submitted in a sterile, plastic leakproof container
Instructions
If using swabs, use only sterile dacron or rayon swabs. Do not use calcium alginate swabs as they may container substances that inhibit PCR testing.
Transport Temperature
Refrigerated
Specimen Stability
Room temperature: 48 hours
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction (PCR)
This test was developed and its performance characteristics were determined by Focus Diagnostics.
Performance characteristics refer to the anlytical performance of the test.
Setup Schedule
Monday-Saturday a.m.
Report Available
Same day
Clinical Significance
Mycoplasma pneumoniae DNA, PCR, is a highly sensitive method to detect the presence of Mycoplasma pneumoniae DNA in clinical specimens.
The diagnosis of Mycoplasma pneumoniae infection should not rely soley upon the result of a PCR assay. A positive result should be determined in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of Mycoplasma pneumoniae DNA at detectable levels in the sample tested and does not exclude the diagnosis of disease.
The diagnosis of Mycoplasma pneumoniae infection should not rely soley upon the result of a PCR assay. A positive result should be determined in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of Mycoplasma pneumoniae DNA at detectable levels in the sample tested and does not exclude the diagnosis of disease.
Performing Laboratory
Focus Diagnostics, Inc.
San Juan Capistrano, CA
