Hydroxyproline

Test Code

HPROL

Alias/See Also

Hydroxyproline, LC/MS, Plasma

Quest Code
37558

CPT Codes
83500

Preferred Specimen

2 mL plasma collected in a sodium heparin (green-top) tube, transferred to a plastic screw-cap vial

Minimum Volume

0.2 mL

Patient Preparation
Collect specimens after an overnight fast (or at least 4 hours after a meal). Nonfasting samples are acceptable for pediatric patients.

Instructions

Please submit a separate, frozen vial for each test ordered requiring a frozen sample.
Separate plasma within 30 minutes of collection.

Transport Temperature

Frozen

Specimen Stability

Room temperature: unacceptable
Refrigerated: 7 days
Frozen: 30 days

Methodology
Liquid Chromatography / Mass Spectrometry (LC/MS)

Setup Schedule

Monday, Wednesday-Friday, Sunday Morning

Report Available

6 days

Clinical Significance

Hydroxyproline is necessary for the construction of collagen. Defects in collagen synthesis lead to easy bruising, internal bleeding, breakdown of connective tissue of the ligaments and tendons, and increased risk to blood vessel damage. Increased loss of hydroxyproline in the urine is generally associated with breakdown of connective tissue, due to disease processes and may also be a manifestation of vitamin C deficiency.

Performing Laboratory
Quest Diagnostics Nichols Institute
San Juan Capistrano, CA

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.