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Ehrlichia chaffeensis DNA Real-Time PCR
Test CodeEHRPCR
Alias/See Also
Ehrlichia chaffeensis PCR Quest Code 11353
Old Quest Code
122021
Old Quest Code
122021
CPT Codes
87798
Preferred Specimen
0.7 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
0.3 mL
Other Acceptable Specimens
0.7 mL whole blood collected in an ACD solution B (yellow-top) tube
0.7 mL CSF submitted in a plastic leakproof container
1 live tick submitted in a plastic leakproof container
1 tick in ETOH, submitted in a plastic leakproof container
0.7 mL CSF submitted in a plastic leakproof container
1 live tick submitted in a plastic leakproof container
1 tick in ETOH, submitted in a plastic leakproof container
Transport Temperature
Refrigerated
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen (whole blood): Unacceptable
Frozen (CSF, tick): 30 days
Refrigerated: 7 days
Frozen (whole blood): Unacceptable
Frozen (CSF, tick): 30 days
Methodology
Polymerase Chain Reaction (PCR)
This test was developed and its performance characteristics have been determined by Quest Diagnostics Nichols Institute. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. Performance characteristics refer to the analytical performance of the test.
Setup Schedule
Monday-Saturday Morning
Report Available
Same day
Clinical Significance
Ehrlichia chaffeensis DNA PCR is a highly sensitive method to detect the presence of Ehrlichia chaffeensis DNA in clinical specimens.
The diagnosis of ehrlichia chaffeensis infection should not rely solely upon the result of PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of Ehrlichia chaffeensis DNA at detectable levels in the sample tested and does not exclude the diagnosis of disease.
Performing Laboratory
Focus Diagnostics, Inc.
San Juan Capistrano, CA
The diagnosis of ehrlichia chaffeensis infection should not rely solely upon the result of PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of Ehrlichia chaffeensis DNA at detectable levels in the sample tested and does not exclude the diagnosis of disease.
Performing Laboratory
Focus Diagnostics, Inc.
San Juan Capistrano, CA
