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Bilirubin, Fractionated
MessageTest performed at York Hospital Laboratory. Do not order on patients less than 14 days old.
Test Code
DBIL
Alias/See Also
Bilirubin, Direct & Indirect Bilirubin Direct Bilirubin Indirect Direct Bilirubin Indirect Bilirubin
CPT Codes
82248
Includes
Bilirubin, Total Bilirubin, Unconjugated Bilirubin, Conjugated Bilirubin, Direct Bilirubin, Delta
Preferred Specimen
0.2 mL serum collected in a serum separator tube (SST)
Other Acceptable Specimens
0.2 mL serum collected in a no additive (red-top) tube, transferred to a plastic screw-cap vial 0.2 mL plasma collected in a lithium heparin (green-top) gel separator tube
Instructions
Protect specimen from prolonged exposure to light and direct sunlight. Separate serum or plasma from the cells within 4 hours of collection.
Transport Temperature
Refrigerated
Specimen Stability
Room temperature: 4 hours
Refrigerated: 7 days
Frozen: 6 months
Refrigerated: 7 days
Frozen: 6 months
Methodology
Spectrophotometry
Setup Schedule
Daily
Report Available
Next Day Available STAT 1/2 Hour
Reference Range
Reference Range | |
---|---|
Bilirubin, Total | 0.2 - 1.3 mg/dl |
Bilirubin, Unconjugated | < 1.1 mg/dl |
Bilirubin, Conjugated | < 0.3 mg/dl |
Bilirubin, Direct | < 0.4 mg/dl |
Bilirubin, Delta | < 0.6 mg/dl |
Clinical Significance
Measurement of the levels of bilirubin is used in the diagnosis and treatment of liver, hemolytic, hematologic, and metabolic disorders, including hepatitis and gall bladder obstruction. The assessment of direct bilirubin is helpful in the differentiation of hepatic disorders. The increase in total bilirubin, associated with obstructive jaundice, is primarily due to the direct fraction. Both direct and indirect bilirubin are increased in the serum with hepatitis.