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Vitamin B12
MessageTest performed at York Hospital Laboratory.
Test Code
B12
CPT Codes
82607
Preferred Specimen
0.3 mL serum collected in a serum separator tube (SST)
Other Acceptable Specimens
0.3 mL serum collected in a no additive (red-top) tube, transferred to a plastic screw-cap vial
0.3 mL plasma collected in a lithium heparin (green-top) gel separator tube
0.3 mL plasma collected in a lithium heparin (green-top) gel separator tube
Instructions
Separate serum or plasma from the cells within 4 hours of collection.
Transport Temperature
Refrigerated
Specimen Stability
Room temperature: 4 hours
Refrigerated: 7 days
Frozen: 4 weeks
Refrigerated: 7 days
Frozen: 4 weeks
Methodology
Chemiluminescence
Setup Schedule
Daily
Report Available
Next Day
Reference Range
239-931 pg/mL
Clinical Significance
Measurement of vitamin B12 is intended to identify and monitor vitamin B12 deficiency. This can arise from the following: (1) defect in the secretion of Intrinsic Factor, resulting in inadequate absorption from food (pernicious anemia); (2) gastrectomy and malabsorption due to surgical resection; and (3) a variety of bacterial or inflammatory diseases affecting the small intestine.
