ACTH Stimulation Test (Cortisol X3)

Test performed at York Hospital Laboratory.

Test Code

Alias/See Also
Cosyntropin (ACTH Stimulation)

CPT Codes

Cortisol, 0 minutes
Cortisol, 30 minutes
Cortisol, 60 minutes

Preferred Specimen
0.2 mL serum collected in a serum separator tube (SST) (each specimen)

Other Acceptable Specimens
0.2 mL serum collected in a no additive (red-top) tube, transferred to a plastic screw-cap vial
0.2 mL plasma collected in a lithium heparin (green-top) gel separator tube

Draw baseline specimen (0 min) before ACTH administration. Administer 250 mcg Cortrosyn by rapid IV push. Draw specimens 30 and 60 minutes after ACTH administration. Clearly mark time drawn on all tubes.

Transport Temperature

Specimen Stability
Room temperature: 4 hours
Refrigerated: 5 days
Frozen: 4 weeks

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed specimen


Setup Schedule
Monday, Thursday

Report Available
4 Days

Clinical Significance
Test is for adrenal function / Addison's disease.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.