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ACTH Stimulation Test (Cortisol X3)
MessageTest performed at York Hospital Laboratory.
Test Code
ACTHST
Alias/See Also
Cosyntropin (ACTH Stimulation)
CPT Codes
82553x3
Includes
Cortisol, 0 minutes
Cortisol, 30 minutes
Cortisol, 60 minutes
Cortisol, 30 minutes
Cortisol, 60 minutes
Preferred Specimen
0.2 mL serum collected in a serum separator tube (SST) (each specimen)
Other Acceptable Specimens
0.2 mL serum collected in a no additive (red-top) tube, transferred to a plastic screw-cap vial
0.2 mL plasma collected in a lithium heparin (green-top) gel separator tube
0.2 mL plasma collected in a lithium heparin (green-top) gel separator tube
Instructions
Draw baseline specimen (0 min) before ACTH administration. Administer 250 mcg Cortrosyn by rapid IV push. Draw specimens 30 and 60 minutes after ACTH administration. Clearly mark time drawn on all tubes.
Transport Temperature
Refrigerated
Specimen Stability
Room temperature: 4 hours
Refrigerated: 5 days
Frozen: 4 weeks
Refrigerated: 5 days
Frozen: 4 weeks
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolyzed specimen
Methodology
Chemiluminescence
Setup Schedule
Monday, Thursday
Report Available
4 Days
Clinical Significance
Test is for adrenal function / Addison's disease.