Fetomaternal Bleed, Flow Cytometry

Test Code

Alias/See Also
Fetomaternal Bleed (Flow Cytometry)
Kleihauer-Betke by Flow Cytometry

Quest Code

CPT Codes

Preferred Specimen
5 mL whole blood collected in a EDTA (lavender or pink-top) tube

Minimum Volume
0.5 mL

Transport Temperature

Specimen Stability
Room temperature: 24 hours
Refrigerated: 4 days
Frozen: unacceptable

Flow Cytometry

Setup Schedule
Monday, Wednesday, Friday a.m.

Report Available
Next day

Clinical Significance
This test quantifies the volume of fetal-maternal hemorrhage (FMH). In Rh-positive mothers it is used solely to establish the volume of fetal hemorrhage into the maternal circulation. In Rh negative mothers it is also used to determine if an increased dose of Rh immune globulin (RhIG, anti-D antibody) is necessary. Based on an assumed total maternal circulatory volume of 5,000 mL, the FMH is calculated by multiplying the %Fetal Cells by 50. Please refer to the most current AABB Technical Manual for RhIG dosage information.

Performing Laboratory
Quest Diagnostics Nichols Institute
Chantilly, VA

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.