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C. difficile DNA amplified probe by PCR, Positive reflex to Toxins A+B
MessageTest performed at York Hospital Laboratory.
Test Code
CDIFFPCR
Alias/See Also
C. diff DNA by PCR
Preferred Specimen
Stool submitted in sterile leakproof container
Transport Temperature
Refrigerated
Specimen Stability
Sterile Container (Unpreserved) Room temperature: 2 days : Refrigerated: 4 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Formed stools, Carey-Blair specimen, Specimens received in any preservative.
Methodology
DNA PCR, Toxins A+B immunoassay
Setup Schedule
Daily
Report Available
Next day, Available STAT, PCR 90-120 Minutes; Toxin additional 60-90 minutes
Reference Range
Negative
Critical Values
Positive
Critical Values
Positive
Clinical Significance
Clostridium difficile is a spore-forming bacterium that is part of the normal intestinal flora. With intestinal overgrowth, C. difficile is the major cause of antibiotic-associated diarrhea and colitis secondary to antimicrobial therapy. C. difficile is the primary cause of pseudomembranous colitis. The organism produces cytotoxins that lead to frequent, foul-smelling, watery stools.