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Von Willebrand Factor Collagen Binding
Test CodeVONCB
Alias/See Also
vWF Collagen Binding Assay
vWF: CBA
Quest Code
10924
vWF: CBA
Quest Code
10924
CPT Codes
83520
Preferred Specimen
1 mL plasma collected in a 3.2% sodium citrate (light blue-top) tube, transferred to a plastic screw-cap vial
Minimum Volume
0.5 mL
Instructions
Please submit a separate, frozen vial for each special coagulation assay ordered.
Centrifuge light blue-top tube 15 minutes at approx. 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the wbc/platelet buffy layer, and place into a plastic vial. Centrifuge aliquoted tube containing plasma for and additional 15 minutes. Transfer platelet-poor plasma into a new plastic vial and freeze within 60 minutes of collection. Plasma must be free of platelets (<10,000/mcL).
Centrifuge light blue-top tube 15 minutes at approx. 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the wbc/platelet buffy layer, and place into a plastic vial. Centrifuge aliquoted tube containing plasma for and additional 15 minutes. Transfer platelet-poor plasma into a new plastic vial and freeze within 60 minutes of collection. Plasma must be free of platelets (<10,000/mcL).
Transport Temperature
Frozen
Specimen Stability
Room temperature: 24 hours
Refrigerated: 24 hours
Frozen: 21 days
Refrigerated: 24 hours
Frozen: 21 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis
Lipemia
Icteric specimen
Received room temperature or refrigerated
Lipemia
Icteric specimen
Received room temperature or refrigerated
Methodology
Enzyme Linked Immunosorbent Immunoassay (ELISA)
This test was performed using a kit that has not been cleared or approved by the FDA. The analytical performance characteristics of this test have been determined by Quest Diagnostics. This test should not be used for diagnosis without confirmation by other medically established means .
Setup Schedule
Monday, Wednesday Night
Report Available
3 days
Clinical Significance
The Collagen Binding Assay (vWF:CBA) is a test procedure that quantifies the binding of vWF to collagen. Collagen binding of vWF is associated with the functionally more important HMW forms of vWF. Therefore, vWF:CBA may correlate more closely with vWF function and bleeding problems than vWF antigen assay (vWF: Ag) which measures total vWF multimers. When used in conjunction with the vWF: Ag assay, vWF:CBA can be used to differentiate between vWD type 1 and type 2 (A and B subtypes) by calculating a ratio of the two assay results (ratio = vWF:CBA/vWFAg).
Performing Laboratory
Quest Diagnostics Nichols Institute
San Juan Capistrano, CA