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EBV DNA, Quant. Real-Time PCR
Test CodeEBVPCR
Alias/See Also
Epstein Barr PCR, CSF or Blood, Quant.
Quest Code
14625
Quest Code
14625
CPT Codes
87799
Preferred Specimen
1 mL serum collected in a serum separator tube (SST)
OR
1 mL CSF collected in a sterile leakproof container
OR
1 mL CSF collected in a sterile leakproof container
Minimum Volume
0.3 mL
Other Acceptable Specimens
1 mL serum collected in a no additive (red-top) tube, transferred to a plastic screw-cap vial
1 mL whole blood collected in an EDTA (lavender-top) tube
1 mL plasma collected in an EDTA (lavender-top) tube, transferred to a plastic screw-cap vial
1 mL whole blood collected in an ACD-B (yellow-top) tube***
1 mL plasma collected in an ACD-B (yellow-top) tube, transferred to a plastic screw-cap vial***
Bone marrow collected in EDTA (lavender-top) tube
Bronchoaveolar lavage, submitted in plastic leakproof container
Eye fluid, submitted in plastic leakproof container
***Blood collected in tubes containing ACD anticoagulatnt will yield results approximately 15% lower when compared to EDTA tubes, due to a dilution effect.
1 mL whole blood collected in an EDTA (lavender-top) tube
1 mL plasma collected in an EDTA (lavender-top) tube, transferred to a plastic screw-cap vial
1 mL whole blood collected in an ACD-B (yellow-top) tube***
1 mL plasma collected in an ACD-B (yellow-top) tube, transferred to a plastic screw-cap vial***
Bone marrow collected in EDTA (lavender-top) tube
Bronchoaveolar lavage, submitted in plastic leakproof container
Eye fluid, submitted in plastic leakproof container
***Blood collected in tubes containing ACD anticoagulatnt will yield results approximately 15% lower when compared to EDTA tubes, due to a dilution effect.
Instructions
Separate serum or plasma from the red cells within 2 hours of collection.
Transport Temperature
Refrigerated
Specimen Stability
Room temperature: 48 hours
Refrigerated: 8 days
Frozen (whole blood, bone marrow): unacceptable
Frozen (all other specimens): 30 days
Refrigerated: 8 days
Frozen (whole blood, bone marrow): unacceptable
Frozen (all other specimens): 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Frozen whole blood or bone marrow
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics Nichols Institute, Chantilly, VA. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Daily a.m.
Report Available
Next day
Clinical Significance
Epstein-Barr Virus DNA, Real-Time PCR is useful in assessing active disease. Central nervous system infections can be diagnosed with CSF specimens.
Performing Laboratory
Quest Diagnostics Nichols Institute
Chantilly, VA
