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5-HIAA (5-Hydroxyindoleacetic Acid), 24-Hour Urine (without Creatinine) (HIAA5)
MessagePatient should avoid food high in indoles: avocado, banana, tomato, plum, walnut, pineapple, and eggplant. Patient should also avoid tobacco, tea and coffee for three days prior to specimen collection.
Test Code
LAB3138
Quest Code
523
Alias/See Also
HIAA5
CPT Codes
83497
Includes
CPT code 81050 may be added at an additional charge for volume measurement
Preferred Specimen
10 mL aliquot from a 24-hour urine that has been preserved with 25 mL 6N HCl, collected in a plastic 24-hour urine container
Patient Preparation
Patient should avoid food high in indoles: avocado, banana, tomato, plum, walnut, pineapple, and eggplant
Minimum Volume
5 mL
Other Acceptable Specimens
Aliquot from a 24-hour urine, unpreserved with a pH ≤6, collected in a plastic 24-hour urine container
Instructions
Collect urine with 25 mL 6N HCl to maintain a pH <3. Urine without preservative is acceptable if pH is <6 and sample is shipped frozen. Keep urine refrigerated during collection if preservatives are not used. Record 24-hour urine volume on test requisition and the urine vial. Record patient's age on test request form and urine vial.
Transport Container
24 hr urine container
Transport Temperature
Room temperature; frozen if unpreserved
Specimen Stability
Preserved urine
Room temperature: 7 days
Refrigerated: 30 days
Frozen: 30 days
Unpreserved urine
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
pH >3.0 and received unfrozen
Methodology
High Performance Liquid Chromatography (HPLC)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Monday-friday
Report Available
3 days
Limitations
Patients with renal disease may display falsely decreased results. Urinary 5-HIAA is increased in patients with malabsorption disorders who also display a larger concentration of urinary tryptophan metabolites. Urinary 5-HIAA is increased in patients with intestinal obstruction and with some noncarcinoid islet cell tumors. Carcinoid syndrome may not cause elevated results, especially if the patient does not have diarrhea.
Reference Range
2-10 Years | ≤8.0 mg/24 h |
>10 Years | ≤6.0 mg/24 h |
Clinical Significance
Values above 10 mg/24 hrs occur in carcinoid syndrome. Slight elevations also occur in nontropical sprue and after reserpine administration.
Drugs which can interfere with testing due to physiologic response: Acetaminophen, Alpha and Beta Blockers, Atenolol, Bromocriptine, Bronchodilators, Clonidine, Digoxin, Isoniazid, L-Dopa, Labetelol, Methyldopa, Monoamine Oxidase Inhibitors, Nitroglycerin, Sympathomimetic Amines, Phenobarbital, Phenothiazines, Phentolamine, Reserprine, Salicylates, Tricyclic Antidepressants.
Performing Laboratory
Quest Diagnostics Nichols Institute-Chantilly VA |
14225 Newbrook Drive |
Chantilly, VA 20151-2228 |